Method for extracting exosomes and exosome proteins

Abstract

The invention discloses a method for extracting exosomes and exosome proteins from human body fluid or culture medium. The method for extracting exosomes and exosome proteins provided by the inventionincludes: extraction materials are added to to-be-extracted solution containing the exosomes, and carrying out extraction to obtain the exosomes; the extraction materials are metallic oxides or function materials modified with the metallic oxides; the metallic oxides and phosphate anions can occur double coordination; and the to-be-extracted solution is body fluid from the human body, diluent ofthe body fluid or cell culture products. Experiment shows that the exosome extraction method of the invention can realize highly-efficient extraction of the exosomes from the human body fluid or the cell culture medium, the extraction time is short, the purity of the obtained exosomes is high, complete exosomes can be obtained by eluent elution, and the used extraction materials can be recycled and reused; in addition, extracting the exosome proteins can reveal that the obtained exosome proteins are more in types and more closely related to exosome functions.

Description

technical field [0001] The invention relates to a method for extracting exosomes and exosome proteins from human body fluid or cell culture fluid in the field of biotechnology. Background technique [0002] Exosomes are a type of vesicle-like structure secreted by mammalian cells through the process of "endocytosis-efflux", with a diameter of about 30-150nm. Exosomes exist not only in cell culture fluid, but also in body fluids such as blood, urine, amniotic fluid, breast milk, semen, saliva, and pleural effusion. Their components include lipid bilayers and cell-derived proteins and RNAs. , DNA and other substances. Almost all cells can produce exosomes, and exosomes from different cell sources contain not only common components related to cell formation, structure, and material transport, but also specific molecules related to the biological functions of the source cells. Exosomes, as an important way of intercellular communication and delivery of a large number of biolog...

AI Technical Summary

Problems solved by technology

The first four methods are based on the physical parameters such as the unique density and size of exosomes, which lack specificity and cannot meet the requirements of specific extraction and analysis of tumor cell exosomes in actual samples.

In particular, human body fluid samples contain a large number of particles such as low-density lipoproteins and chylomicrons. The particle size and density of these particles are close to those of exosomes. Most methods cannot separate them from exosomes, so that exosomes Interfering with subsequent analysis

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