Composite tendon anti-adhesion film and preparation method and application thereof

An anti-adhesion film and tendon technology, applied in the field of biomedical materials, can solve the problems of unavoidable growth of fibroblasts, unfavorable nutrient transport, poor adhesion between the film and the body, etc., to promote endogenous healing function and suitable for degradation Effect of time, effective isolation

Inactive Publication Date: 2019-10-22
天新福(北京)医疗器材股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are four types of silica gel film, sodium hyaluronate film, chitin film and polylactic acid film on the market. Their anti-adhesion mechanisms are different. Although they can all play a role in preventing adhesion to a certain extent, they all have their own shortcomings. , such as only have good biocompatibility, adhesion, degradability and other advantages, but for the time of tendon repair, the degradation is too fast; some products have a three-dimensional porous structure that can promote endogenous healing and help ensure nutrients Transport, but cannot prevent fibroblasts from growing into
[0004] It has been reported in the literature that chitosan can inhibit the proliferation of tendon sheath fibroblasts, epitenon and endotoneurosis cells in the process of tendon repair and has good hemostatic effect, but the membrane has poor adhesion to the body and is difficult to operate during surgery. The package is fixed, needs to be stitched and the stitching is not strong enough
The degradation time of polylactic acid is close to that of tendon repair, and it can prevent peritendon adhesion and fully play the role of barrier. However, it is made by casting film method, and it has no pores, which is not conducive to the transportation of nutrients, and its flexibility is poor, and its adhesion performance is also poor.

Method used

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  • Composite tendon anti-adhesion film and preparation method and application thereof
  • Composite tendon anti-adhesion film and preparation method and application thereof
  • Composite tendon anti-adhesion film and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Embodiment 1, preparation of composite tendon anti-adhesion film

[0052] 1. Preparation of polylactic acid chitosan nanofiber membrane

[0053] Dissolve polylactic acid (molecular weight 10kDa) and chitosan (molecular weight 50kDa) in solvent (trifluoroacetic acid) to obtain electrospinning solutions with final concentrations of 240g / L and 20g / L respectively, and then carry out electrostatic spinning according to the following process parameters Spinning to obtain PLA-chitosan nanofibrous membrane:

[0054] Needle No. 20, electrostatic high voltage 20kV, propulsion speed 0.15mm / min, receiving distance (ie polar distance) 22cm, spinning time 120min, spun fiber thickness 0.1-0.2mm.

[0055] 2. Collagen Gel Preparation

[0056] Using 0.5mol / L glacial acetic acid solution as a solvent, a collagen gel with a type I collagen concentration of 70g / L was prepared.

[0057] 3. Preparation of composite tendon anti-adhesion membrane

[0058] Apply the collagen gel prepared in ...

Embodiment 2

[0059] Embodiment 2, preparation of composite tendon anti-adhesion film

[0060] 1. Preparation of polylactic acid chitosan nanofiber membrane

[0061] Dissolve polylactic acid (molecular weight 10kDa) and chitosan (molecular weight 10kDa) in solvent (trifluoroacetic acid) to obtain electrospinning solutions with final concentrations of 270g / L and 30g / L respectively, and then carry out electrostatic spinning according to the following process parameters Spinning to obtain PLA-chitosan nanofibrous membrane:

[0062] Needle No. 20, electrostatic high voltage 20kV, advancing speed 0.15mm / min, receiving distance 22cm, spinning time 90min, spun fiber thickness 0.1-0.2mm.

[0063] 2. Collagen Gel Preparation

[0064] With embodiment 1 step 2.

[0065] 3. Preparation of composite tendon anti-adhesion membrane

[0066] Apply the collagen gel prepared in step 2 on the collagen layer (amount of 0.8ml / cm 2 Anti-adhesion film), after bonding the nanofiber film in step 1, drying at 25...

Embodiment 3

[0067] Embodiment 3, preparation of composite tendon anti-adhesion film

[0068] 1. Preparation of polylactic acid chitosan nanofiber membrane

[0069] Dissolve polylactic acid (molecular weight 10kDa) and chitosan (molecular weight 3kDa) in solvent (trifluoroacetic acid) to obtain electrospinning solutions with final concentrations of 270g / L and 30g / L respectively, and then carry out electrostatic spinning according to the following process parameters Spinning to obtain PLA-chitosan nanofibrous membrane:

[0070] No. 20 needle, electrostatic high voltage 20kV, propulsion speed 0.15mm / min, receiving distance 22cm, spinning time 80min, spinning fiber thickness 0.1-0.2mm, polylactic acid chitosan nanofiber membrane such as image 3 and 4 shown.

[0071] 2. Collagen Gel Preparation

[0072] With embodiment 1 step 2.

[0073] 3. Preparation of composite tendon anti-adhesion membrane

[0074] With embodiment 1 step 3.

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Abstract

The invention discloses a composite tendon anti-adhesion film and a preparation method and application thereof. The composite tendon anti-adhesion film comprises an inner layer and an outer layer, wherein the inner layer is a collagen layer, the outer layer is a nanofiber film, and the inner layer and the outer layer are bonded by collagen gel. The nanofiber film is prepared by electrostatic spinning of polylactic acid and chitosan, which are used as raw materials. The mass ratio of polylactic acid to chitosan is (5-13):1, preferably (5.4-12):1, more preferably (8-10):1, and more preferably 9:1. The product of the invention has good adhesion, requires no suture to reduce inflammatory reaction, has more suitable degradation time, can fully exert its physical separation function and better hemostasis function in tendon repair process, and can realize nutrient transportation, and has ideal effects close to tendon anti-adhesion products.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a composite tendon anti-adhesion film and its preparation method and application. Background technique [0002] Adhesions are abnormal structures where fibrous bands of connective tissue join together with adjacent tissues or organs. Adhesions are prone to occur in the process of tissue healing after surgery, which affects the recovery of the performance and function of the surgical site. Especially in the tendon area, adhesion problems are very common, mainly caused by fibroblasts of the hyperplastic connective tissue membrane around the tendon contacting the tendon. The recovery time of tendon injury is relatively long, and the adhesion phenomenon will seriously affect the recovery of normal function of tendon. [0003] Products that reduce the adhesion formed by tissues around the tendon (ie exogenous healing), promote the division and proliferation of tendon cells themse...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/06A61L31/14D04H1/728
CPCA61L31/042A61L31/044A61L31/06A61L31/148D04H1/728C08L67/04C08L5/08
Inventor陈刚杨洪阳董会张伊骁刘麟孙冰冰
Owner天新福(北京)医疗器材股份有限公司