Mouse nerve retrograde tracing mark component

Abstract

A mouse nerve retrograde tracing mark component comprises a cylinder tank body and an upper cover. A shape of the cylinder tank body is a cylindrical shape, a cross section of the cylinder tank body is a major arc, an upper cover is a curved plate and the cross section is an inferior arc, the upper cover fastens the cylinder tank body, and the upper cover and the cylinder tank body form a completecylindrical shape. Single and multiple retrograde tracing marking of mouse nerves can be realized so that direct observation of a mutual relation between different sources of motor neuron pools or neuronal subgroups in a spinal cord becomes possible.

Description

technical field [0001] The invention belongs to the technical field of neurobiological science experiments, and in particular relates to a mouse nerve retrograde trace marker component. Background technique [0002] Neuroretrograde tracing is a common technique in neurobiological research, which can be used to determine the origin, quantity and distribution of neuronal somata projecting to specific target areas. There are many labeling methods for nerve retrograde tracing, including: soaking the nerve stump in a tracer (ie, dye), or injecting the dye directly into the nerve trunk or target muscle. However, intramuscular injection of the dye using either the neural stem or the target results in less efficient labeling of neurons and greater variability in the amount of labeling. In addition, nerve stump soaking methods, such as the traditional small pool method and the recently appeared silica gel cap method, the small pool method and the silica gel cap method are described ...

AI Technical Summary

Problems solved by technology

However, intramuscular injection of dyes using neural stems or targets results in less efficient labeling of neurons and greater variability in the amount of labeling

In addition, nerve stump soaking methods, such as the traditional small pool method and the recently appeared silica gel cap method, the small pool method and the silica gel cap method are described in "Retrograde labeling of regenerating motor and sensory neurons using silicon caps” has been introduced, but it is still difficult to operate for single tracer labeling of mouse nerves, and it is even more difficult to achieve for multiple tracer labels (compared with single tracer technology, multiple tracer The technique allows for a more intuitive view of the interrelationships between different neuronal subpopulations within the spinal cord, thereby greatly reducing the number of experimental mice required

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