Dried orange peel reference extract, preparation method and application thereof

A tangerine peel extract and extract technology, applied in the field of chemistry, can solve the problems of high detection cost and incomplete detection standards

Inactive Publication Date: 2019-10-25
广州万正药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to solve the above-mentioned technical problems, the object of the present invention is to provide a kind of in order to solve the above-mentioned technical problems, the detection standard that the present invention exists in the detection of tangerine peel preparation or tangerine peel medical material is not comprehensive, the problem of using multiple reference substances to detect high cost, the present invention Provided is

Method used

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  • Dried orange peel reference extract, preparation method and application thereof
  • Dried orange peel reference extract, preparation method and application thereof
  • Dried orange peel reference extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0189] 1 Thin-layer chromatography screening of raw materials

[0190] 1.1 Preparation of the test product

[0191] Preparation of chemical reference substance solution: take hesperidin, neohesperidin, naringin, nobiletin and tangeretin reference substances and accurately weigh them, and prepare 0.1 mg / ml solutions with methanol respectively.

[0192] Preparation of medicinal materials for test solution: Xinhui Gujing tangerine peel (year: 2013 Figure 15 ); Xinhui Gujing Tangerine Peel (Year: 2010 Figure 16 ); Xinhui Gujing Chenpi (Year: 2006 Figure 17 ) each 0.5g of medicinal material powder, respectively add 5ml of methanol in a 15ml centrifuge tube, soak at room temperature for 15 minutes, take it out after 15 minutes of ultrasonic treatment (power 500w), centrifuge for 10 minutes (rotating speed is 4000 rpm), take the supernatant Obtain the test solution of the medicinal material, or directly pass through a 0.22 μm filter membrane without centrifugation to obtain the...

Embodiment 2

[0270] Chromatographic analysis of tangerine peel reference extract (ERS):

[0271] 1 TLC analysis

[0272] 1.1 Sample preparation

[0273] Chemical reference substance (CRS) solution: take hesperidin, neohesperidin, naringin, nobiletin and tangeretin reference substances and weigh them accurately, and prepare 0.1 mg / ml solutions with methanol respectively.

[0274] Contrast extract (ERS) solution: take an appropriate amount of tangerine peel reference extract from Example 1 and accurately weigh it, add methanol for ultrasonic extraction for 30 minutes, constant volume, prepare a solution with a concentration of 8 mg / ml, pass through a 0.22 μm filter membrane, and take the filtrate as a contrast Extract solution. 1.2 TLC detection

[0275] 1.2.1 Flavonoid glycosides

[0276] Thin layer plate: TLC G60 ordinary silica gel prefabricated plate (Merck).

[0277] Spotting: chemical reference solution: 5 μl, strip spotting.

[0278] Control extract solution: 1 μl, strip spottin...

Embodiment 3

[0310] Adopt 70% ethanol to replace methanol to carry out in the step one among the embodiment 1, the content of hesperidin in the orange peel extract obtained at last is 3.15% (far less than 14%~22% of the present invention), thus it can be seen that its The value is significantly lower than the yield of methanol extraction, and only by increasing the extraction amount or frequency can it be possible to adjust the hesperidin content to reach the deployment standard range.

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Abstract

The invention discloses a dried orange peel reference extract, a preparation method and application thereof. The extract is yellow green dry powder and consists of 65% to 84% of a dried orange peel extract and 16% to 35% of a flow aid in percentages by weight; the dried orange peel extract comprises the ingredients of flavonoid glycoside and flavonoid aglycones; and neohesperidin or naringin is not contained in flavonoid glycoside. The dried orange peel reference extract is high in uniformity, stable in character and uniform, and can be used for mass control of dried orange peel medicinal materials and dried orange peel preparations for qualitative and quantitative assay determination of effective compounds.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a comparison extract of tangerine peel and a preparation method thereof. Background technique [0002] Drug testing is an important means to verify the quality of drugs. With the widespread use of instrumental analysis, more and more drug reference materials are used. In drug inspection work, reference substances are often used as a special reference for checking the quality of drugs, and as a comparison standard for determining the authenticity of drugs. Reference substances play a very important role in drug testing. [0003] In the current standard of traditional Chinese medicine, the quality control mode of a single component is difficult to reflect the quality of traditional Chinese medicine, and the method of comprehensive quality control using multiple components or characteristic chromatographic peaks has attracted more and more attention. However, the shortage of c...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/90G01N30/02
CPCG01N30/02G01N30/06G01N30/90G01N2030/027G01N2030/042G01N2030/062
Inventor 郭隆钢许舜军许艺镌赵岳锐
Owner 广州万正药业有限公司
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