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An ink cartridge and biological technology, which is applied in the fields of biochemical equipment and methods, microsphere preparation, biochemical instruments, etc., can solve the problems of low bio-brick preparation efficiency and low activity of bio-bricks.
Pending Publication Date: 2019-10-29
SICHUAN REVOTEK CO LTD
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[0009] In the process of realizing the present disclosure, the inventors found that the production efficiency of bio-bricks and the low activity of bio-bricks existed in the preparation of bio-bricks using the technology of the above application
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[0531] The microsphere preparation device of this embodiment can be used to produce microspheres for cells from different sources, and the microspheres can be used as the nucleus of the biobrick. For example, microspheres can be made for cells of human origin, porcine origin, monkey origin or custom origin. For cells from different sources, the pressure and other control parameters used in the preparation of microspheres are different, because the cells of different sources have different sizes and the cells can withstand different shear forces. The method for preparing biobrick nuclei will be described below by taking the preparation of monkey-derived cell microspheres as an example. The preparation method of bio-bricks from other cells is similar to that of monkey-derived cells. Due to the different cell sizes, types, shear stress and microsphere sizes, there are differences in the control of pressure parameters, but the parameter range is generally the same. . The prepara...
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Abstract
The invention provides a biological ink cartridge, a biological ink cartridge assembly, microsphere preparation equipment, shell layer assembly equipment, a biological brick preparation instrument, abiological ink preparation instrument and a biological ink preparation system. The biological ink cartridge includes an ink cartridge shell, an isolation wall, a first ink cartridge inlet and a firstink cartridge outlet, a second ink cartridge inlet and a second ink cartridge outlet, and an inner flow channel, wherein the ink cartridge shell is provided with a cavity; the isolation wall is arranged in the cavity, and the cavity is separated into a first containing cavity and a second containing cavity which are isolated from each other; the first ink cartridge inlet and the first ink cartridge outlet are formed in the ink cartridge shell and communicate with the first containing chamber and are isolated from the second containing chamber in a fluid mode; the second ink cartridge inlet andthe second ink cartridge outlet are formed in the ink cartridge shell and communicate with the second containing cavity; and the inner flow channel is located in the second containing cavity and communicates the first containing cavity and the first ink cartridge outlet. The biological ink cartridge, the biological ink cartridge assembly, the microsphere preparation equipment, the shell layer assembly equipment, the biological brick preparation instrument, the biological ink preparation instrument and the biological ink preparation system are beneficial to improving the preparation efficiencyof microsphere preparation, shell layer assembly and biological bricks, further, the biological ink cartridge, the biological ink cartridge assembly, the microsphere preparation equipment, the shelllayer assembly equipment, the biological brick preparation instrument, the biological ink preparation instrument and the biological ink preparation system are also beneficial to improving activity ofbiological brick intermediate products and the biological bricks.
Description
technical field [0001] The present disclosure relates to the technical field of bioprinting, and in particular to a bio-ink cartridge, a bio-ink cartridge assembly, a microsphere preparation device, a shell assembly device, a bio-brick preparation device, a bio-ink preparation device and a bio-ink preparation system. Background technique [0002] 3D bioprinting technology is a biological product preparation technology that has emerged in recent years, and it has been used to construct complex tissues and organs. An important direction of 3D bioprinting technology is that the bioink used for printing can meet the printing requirements. In the known bioprinting technology, the cells in the bioink used lack mechanical protection. When the cells are used for 3D bioprinting, the cells are easy to be damaged or die due to external pressure or shear force. Limit the application of bioprinting technology. [0003] In the Chinese patent application submitted by the applicant of the...
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