Tilapia feed additive for reducing pro-inflammatory response and enhancing immune response, preparation method and application thereof

A technology for enhancing immune response and feed additive, applied in the field of tilapia feed additive and its preparation, can solve problems such as water pollution and excessive excrement, and achieve the effect of promoting immune response, leaving no muscle residue, and enhancing healthy breeding.

Active Publication Date: 2022-06-07
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, tilapia eats vigorously in summer and excretes a lot, causing serious water pollution

Method used

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  • Tilapia feed additive for reducing pro-inflammatory response and enhancing immune response, preparation method and application thereof

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preparation example Construction

[0025] The present invention provides a method for preparing the tilapia feed additive, comprising the following steps:

[0026] 1) Mix the eleutherococcus stalks, loquat leaves, triptolide, hawthorn and mulberry leaves, and cook them with water to get the decoction solution;

[0027] 2) The decoction solution is concentrated, the resulting concentrate is alcohol deposited, the solid-liquid is separated, and the liquid phase is collected to obtain feed additives.

[0028] The present invention is a mixture of eleutherococcus stalks, loquat leaves, triptolide, hawthorn and mulberry leaves, decoction with water to give decoction solution.

[0029] In the present invention, the volume ratio of the total mass of Chinese herbal medicines to water is preferably 0.8 to 1.2g: 0.8 to 1.2mL, more preferably 1g: 1mL. The decoction temperature is preferably 90 ~ 100 ° C, more preferably 95 ° C. The decoction time is preferably 1 to 2h, more preferably 1.5h. After decoction, it is preferable t...

Embodiment 1

[0038] Preparation method for reducing the pro-inflammatory response of tilapia adults in flow-through tank culture and enhancing the immune response of feed additives, comprising the following steps:

[0039]Accurately weigh Eleutherocococcus stalk 30g, loquat leaf 20g, Triptycea 20g, hawthorn 15g and mulberry leaf 15g, after mixing, according to the proportion of adding 1L of water per 1kg of Chinese herbal medicine, steaming at 95 °C for 1.5h, solid-liquid separation, collection of aqueous phase. Dry the moisture at 55 °C in an oven for 3 h to obtain the concentrate. The concentrate is well settled with 95% ethanol. Extraction filtration, filtrate at 60 °C, -0.08Mpa conditions under reduced pressure recovery, to obtain extract. The resulting extract was dried in a 45 °C oven for 2 h and crushed into dry powder.

Embodiment 2

[0041] A set of feed compatibility and implementation methods to reduce the pro-inflammatory response and enhance the immune response of tilapia adults in flow tank culture, including the following steps:

[0042] Accurately weigh eleuthero stem 30 g, loquat leaf 20 g, triptolide 20 g, hawthorn 15 g and mulberry leaf 15 g. The above Chinese herbal medicines are mixed and decocted with water, add 1 L of purified water per 1kg of Chinese herbal medicines, and cook at 100 °C for 1.0h. Dry the moisture at 55 °C in an oven for 3 h to obtain a concentrate. According to the volume ratio of ethanol aqueous solution to concentrate 4:1, add an appropriate amount of 95% ethanol to the alcohol sink tank to submerge the concentrate. Extraction filtration (the pore size of the suction membrane is 3 μm), the filtrate is recovered under reduced pressure at 65 °C and -0.08Mpa, and the extract with a relative density of 1.03~1.12 is obtained. The resulting extract was dried in a 45 °C oven for 2 h ...

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Abstract

The invention provides a tilapia feed additive for reducing proinflammatory response and enhancing immune response, a preparation method and application thereof, and belongs to the technical field of aquaculture. Tilapia feed additives for reducing pro-inflammatory response and enhancing immune response, comprising the following components in parts by weight: 25-35 parts of Acanthopanax stems, 15-25 parts of loquat leaves, 15-25 parts of Tripterygium wilfordii, 10-35 parts of hawthorn 20 parts and 10-20 parts of mulberry leaves. The invention also provides a tilapia feed containing the tilapia feed additive. Tilapia feed additive or the application of said tilapia feed in tilapia culture, said application can effectively reduce the pro-inflammatory response of tilapia head kidney tissue and spleen tissue, enhance its immune response ability, thereby Improve its ability to resist streptococcal infection.

Description

Technical field [0001] The present invention belongs to the field of aquaculture technology, relates to the field of culture management and feed feeding technology, specifically relates to tilapia feed additives that reduce the pro-inflammatory response and enhance the immune response and preparation methods and applications thereof. Background [0002] Circulating water culture in flow tanks is to change the "open free culture" of traditional ponds into "intensive captivity", so that "still water" ponds can achieve "flowing water" fish farming. Through mechanical wave creation, a circular water flow is formed in the entire large pond, and the water flow through the tank can be fed intensively in the "runway", and the excrement can be concentrated in a tank for unified treatment, thus playing a role in purifying the water quality of the pond. Environmental stress alters the body's non-specific immunity, causing inflammation and damage to cells in fish. In recent years, China has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/80A23K10/30A23K40/10A01K61/13A61K36/734A61P29/00A61P37/04A61P31/04
CPCA23K50/80A23K10/30A23K40/10A01K61/13A61K36/254A61K36/73A61K36/37A61K36/734A61K36/605A61P29/00A61P37/04A61P31/04A61K2236/331A61K2236/51A61K2236/53A61K2300/00Y02A40/81
Inventor 强俊何杰徐跑陶易凡包景文朱昊俊曹哲明
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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