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Application of ehd2 autoantibody detection reagent in the preparation of lung cancer screening kit

A technology of autoantibodies and kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of no EHD2 protein, no lung cancer, etc., and achieve good application prospects, low patient harm, and effective Effect of screening

Active Publication Date: 2021-10-01
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant reports on EHD2 protein autoantibodies, and there is no prior art related to lung cancer

Method used

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  • Application of ehd2 autoantibody detection reagent in the preparation of lung cancer screening kit
  • Application of ehd2 autoantibody detection reagent in the preparation of lung cancer screening kit
  • Application of ehd2 autoantibody detection reagent in the preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Relationship Between EHD2 Autoantibodies in Plasma and Lung Cancer

[0026] 1. Clinical data

[0027] A total of 29 lung cancer patients and 29 healthy controls were selected. The basic information is as follows:

[0028] Basic Information lung cancer patients healthy control number of people 29 29 age 58±10.5 42±8.9 male ratio 20(69.0％) 13(44.8％)

[0029] 2. Detection principle

[0030] HuProt TM EHD2 protein is immobilized on the human protein custom chip. After incubation with serum, EHD2 autoantibodies (mainly including IgG, IgM antibodies, and some other types of antibodies) in the serum will bind to it, and unbound antibodies and other proteins will be removed by washing. Then use anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green) to detect, and read the signal through a fluorescent scanner. The strength of the signal i...

Embodiment 2

[0047] Embodiment 2 The composition of detection kit of the present invention and its using method

[0048] 1. Kit composition

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Abstract

The invention relates to the field of in vitro diagnostic reagents, in particular to the use of EHD2 autoantibody detection reagents in the preparation of lung cancer screening kits. The present invention finds for the first time that the autoantibody level of EHD2 protein in serum of patients with lung cancer is significantly higher than that of healthy patients. The invention uses the reagent for detecting EHD2 protein autoantibody to prepare a lung cancer screening kit, which can realize effective screening of lung cancer.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of EHD2 autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57423G01N33/57484G01N33/6884
Inventor 张立李为民
Owner WEST CHINA HOSPITAL SICHUAN UNIV