Application of umbilical cord mesenchymal stem cell conditioned medium

A technology of conditioned medium and stem cells, applied in the field of conditioned medium of umbilical cord mesenchymal stem cells, can solve the problems of inconvenient storage and transportation, immune rejection, etc., and achieve low impact of environmental factors, sufficient tissue sources, product stability and safety. high sex effect

Pending Publication Date: 2019-11-22
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the direct use of stem cell therapy has the disadvantages of inconvenient storage and transportation as well as immune rejection
There is no report on the use of conditioned medium of mesenchymal stem cells in the treatment of ovarian insufficiency

Method used

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  • Application of umbilical cord mesenchymal stem cell conditioned medium
  • Application of umbilical cord mesenchymal stem cell conditioned medium
  • Application of umbilical cord mesenchymal stem cell conditioned medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of embodiment 1.UCMSC-CM

[0048] UCMSC (third generation) was provided and identified by Beijing Stem Cell Bank, Institute of Zoology, Chinese Academy of Sciences. Seed UCMSCs in a 100mm culture dish, add 10ml UCMSC medium (DMEM / F12, 10% FBS, GlutaMAX, penicillin-streptomycin, 4ng / ml basic FGF) for culture, when the cell confluency reaches 80%, discard the culture culture medium, washed 3 times with sterile PBS, added 6ml of serum-free medium (DMEM / F12) to each 100mm culture dish, placed the cells in a 37°C, 5% CO2 cell incubator for 24 hours, and collected the culture medium after the culture was over. And centrifuge, 1500rpm, centrifuge for 5 minutes to remove cell debris, aliquot into 1ml centrifuge tubes, store at -80°C for later use, and the collected conditioned medium UCMSC-CM comes from the 4th-6th generation UCMSC.

Embodiment 2

[0049] Example 2. Establishment of Ovarian Injury Model in Mice

[0050] The mouse ovary at 5 days after birth (P5) is mainly composed of primordial follicles and primary follicles, which are extremely sensitive to toxic agents, so mice at 5 days after birth are ideal for studying the mechanism of chemotherapy drug-induced death of immature follicles time. P5 mice were given a single intraperitoneal injection of cisplatin, the dose of cisplatin injection was 5 mg / kg body weight, and the mice in the control group were injected with the same amount of normal saline. The mice were killed by cervical dislocation 4 days after injection of cisplatin or normal saline, and the ovaries were collected. And perform fixed embedding section ( figure 1 A).

[0051] Results: Compared with the control group, the primordial follicles in the ovaries of the cisplatin treatment group were significantly reduced ( figure 1 B). Therefore, in the following in vivo experiments in mice, we selected...

Embodiment 3

[0052] Example 3. The protective effect of UCMSC-CM on ovarian damage caused by cisplatin in mice

[0053] In order to explore the protective effect of human umbilical cord mesenchymal stem cell conditioned medium on cisplatin-induced ovarian damage in mice, CD-1 mice born 5 days old (P5) (purchased from Speifu (Beijing) Laboratory Animal Technology Co., Ltd. , mother mice feeding) 80, weighing about 2.8g body weight. Divide P5 mice into four groups, blank control group: continuous intraperitoneal injection of common medium for 4 days; cisplatin group: a single intraperitoneal injection of 5 mg / kg body weight of cisplatin on the first day of treatment, and then continuous intraperitoneal injection of common medium 3 days; conditioned medium group: continuous intraperitoneal injection of conditioned medium for 4 days; cisplatin + conditioned medium group: a single intraperitoneal injection of 5 mg / kg body weight of cisplatin on the first day of treatment, and then continuous in...

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Abstract

The invention relates to an application of an umbilical cord mesenchymal stem cell conditioned medium, in particular to a protective effect of the human umbilical cord mesenchymal stem cell conditioned medium or freeze-dried powder prepared by the human umbilical cord mesenchymal stem cell conditioned medium on ovarian injury, ovarian senescence and the like.

Description

technical field [0001] The present invention relates to the use of umbilical cord mesenchymal stem cell conditioned medium, in particular, the present invention relates to the protective effect of human umbilical cord mesenchymal stem cell conditioned medium or freeze-dried powder prepared therefrom on ovarian damage, ovarian aging and the like. Background technique [0002] Stem cells are a type of undifferentiated cells with self-replication ability and multi-directional differentiation potential, and have the potential function of regenerating various tissues and organs of the human body, so they are also called "universal cells" by the medical field. A number of studies have confirmed that stem cells can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, heart muscle, endothelial and other tissue cells under specific induction conditions. Not only that, stem cells are continuously subcultured and frozen After preservation, the initial multi-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/19A61P15/08C12N5/0775
CPCA61K9/19A61K35/28A61P15/08C12N5/0668
Inventor 王红梅闫龙杨晓葵洪黎明吴骏郝捷
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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