Experimental method for the simultaneous recording of monophasic and biphasic action potentials in the neural trunk

Abstract

An experimental method for simultaneous recording and research of single-biphasic action potentials in nerve trunks, comprising the following steps: (1), first preparing isolated nerve trunks, forming an O-ring in the middle of the nerve trunks, and using thin wires to connect the back of the nerve trunks. The intersection nodes of the first half and the first half are bound, and then the O-ring is cut off; (2), the nerve trunk is placed in a multi-electrode nerve specimen box, and the intersection node of the nerve trunk is located at the second recording electrode and the third recording electrode (3), electrify the first stimulating electrode and the second stimulating electrode, record the biphasic AP between the first recording electrode and the second recording electrode, and record the AP between the first recording electrode and the third recording electrode (4) Analyze and compare the biphasic AP and single-phase AP obtained in step (3). The present invention can accurately compare and analyze the similarities, differences and internal connections between the biphasic AP and monophasic AP at the same moment between the same two points of the nerve trunk under the same stimulation condition.

Description

technical field [0001] The invention belongs to the technical field of physiological experiment research, and in particular relates to an experimental method for simultaneous recording and research of single-biphasic action potential of a nerve trunk. Background technique [0002] Nerve trunk (nerve trunk) is covered by epineurium and contains many nerve fibers. Nerve fibers realize rapid long-distance transmission of information by generating and conducting action potentials. Nerve trunk action potentials (action potentials, AP) are the comprehensive electrical signals (compound action potentials) that are generated by multiple nerve fibers in the nerve trunk and transmitted to the surface of the nerve trunk to be recorded. The classic content of learning experiment teaching and research. [0003] According to different recording conditions, neural trunk action potential can be divided into neural trunk biphasic action potential and neural trunk monophasic action potentia...

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Problems solved by technology

[0006] Similarly, since existing experimental methods cannot accurately compare and analyze the characteristics of biphasic AP and monophasic AP between the same two points under the same stimulation conditions, the neural trunk biphasic AP given in existing related books The peak value of the waveform and the single-phase AP waveform are all of the same height. For related content, please refer to "Analysis of Physiological Hundred Words", edited by Cui Gengyin, presided over by Gu Shuangzhen and Guan Zhenlong, China Press of Traditional Chinese Medicine, Beijing, 2005, P34-36; "Animal Physiology", 3rd edition, edited by Chen Shouliang, 2005, P29-31, Peking University Press; "Experimental Physiology", edited by Wang Tinghuai, Yang Huiling, Wang Xuelan, 2014, P138-139, Higher Education Press; Experimental Science (Bilingual Textbook), edited by Xie Keming, Wang Guoqing, Jiang Xinghong, Sheng Rui, etc., 2014, P113-114, Higher Education Press; "Experimental Course of Medical Functional Science", 4th edition, edited by Hu Huanzhong, Mou Yangling, etc. , 2016, P101, Science Press; etc.

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