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Screening method of composite microbial inoculum through low-temperature straw composting and application

The technology of compound inoculum and compound microbial inoculum, which is applied in the field of microbiology, can solve the problem that the screening method and application of compound inoculant for low temperature straw composting has not yet been reported, and can improve the physical and chemical properties of soil, improve the micro-ecosystem, The effect of enhancing breathability

Active Publication Date: 2019-12-03
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In a word, the screening method and application of a low-temperature straw composting compound bacterial agent have not been reported yet.

Method used

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  • Screening method of composite microbial inoculum through low-temperature straw composting and application
  • Screening method of composite microbial inoculum through low-temperature straw composting and application
  • Screening method of composite microbial inoculum through low-temperature straw composting and application

Examples

Experimental program
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Effect test

preparation example Construction

[0028] The preparation method of composite microbial bacterial agent:

[0029] The yeast and Bacillus subtilis are placed in the culture medium at a strain ratio of 2:3, and cultured for 12-28 hours at a pH of 4.8-7.2 and a temperature of 5-15°C to obtain a compound microbial bacterial agent.

[0030] An application of a low-temperature straw composting compound bacterial agent, and a method for preparing fertilizer using the compound microbial bacterial agent:

[0031] (1) Put yeast H4 and Bacillus subtilis TY-1 in the culture medium according to the strain ratio of 2:3, and cultivate them for 12-28 hours under the conditions of pH=4.8-7.2 and temperature 5-15°C to obtain compound microbial agent;

[0032] (2) The composition of the medium, by weight percentage, is 2-3% soluble starch, 0.5-1% peptone, (NH 4 ) 2 SO 4 0.5~1%, K 2 HPO 4 0.07~0.1%, KH 2 PO 4 0.03~0.5%, MgSO 4 ·7H 2 O 0.01~0.05%, the balance is water;

[0033] The composition and weight ratio of the ...

Embodiment example

[0040] CK: Adjust the moisture content of 1 ton of corn stalks to 50%.

[0041] H4: Add 4kg of bacterial agent H4 on the basis of CK.

[0042] TY-1: Add TY-1 6kg on the basis of CK.

[0043] H4+TY-1: Add 10kg of H4+TY-1 complex microbial agent (2:3) on the basis of CK.

[0044] Composting time: 2019.3.13-2019.4.25.

[0045] Composting location: Shenyang.

[0046] Specific process: each pile of compost is 1000kg, and the corn stalks are neatly stacked parallel to the ground. When each rise is 20cm, a little inoculation agent is sprinkled on the surface, and at the same time, it is stepped on tightly, and the pile height is kept at 1.5. The outer surface is covered with agricultural plastic film , accumulated for a total of 45 days.

[0047] Index: Determination of straw composting temperature and straw lignin, cellulose, and hemicellulose contents in different periods.

[0048] The results showed that: under the condition of average spring temperature of 6°C, the average t...

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Abstract

The invention relates to a screening method of composite microbial inoculum through low-temperature straw composting and an application. The composite microbial inoculum comprises bacillus subtilis and microzymes, and is preserved in the China General Microbiological Culture Collection Center, the preservation No. is CGMCC No.18001\CGMCC No.18002, and the preservation date is 19 June 2019. High-efficiency low-temperature cellulose-decomposition bacteria are separated and screened from soil of different regions, and the adaptability of the composite microbial inoculum to temperature, a carbon source, pH and the like is researched. Through combination of bacterial strains in different proportions, the composite microbial inoculum is formed, and the composite microbial inoculum is added to low-temperature corn straw compost, so that the progress that corn straw becomes thoroughly decomposed is accelerated, the influence of straw incineration in Northeast China to environment is reduced, and a solid foundation is established for sustainable development of agriculture.

Description

technical field [0001] The invention relates to the application of a low-temperature straw heap rotting composite bacterial agent for low-temperature decomposition of corn stalks, which belongs to the field of microbiology, and more specifically relates to a screening method and application of a low-temperature straw heap rotting composite bacterial agent. Background technique [0002] As a large agricultural country, our country has a high crop yield, and the output of crop straw is also very large, ranking first in the world. Crop straw has also become a huge renewable resource. The prospect of comprehensive utilization of straw is very broad, but this This resource is difficult to be utilized, and most of the straw is burned, which not only causes waste of resources, but also causes serious environmental pollution. Studies have shown that burning straw in a large area on the soil surface will reduce the number and types of soil microorganisms, and the diversity of microbi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/02C05F11/08C12R1/125
CPCC12N1/20C12N1/16C12N1/02C05F11/08Y02W30/40
Inventor 何志刚隋国民许月凡刘惠屿刘艳宋艳雨
Owner LIAONING ACAD OF AGRI SCI
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