Erbb2/her2 mutations in the transmbrane or juxtamembrane domain
A transmembrane and near-membrane technology, applied in the determination/testing of microorganisms, peptide/protein components, medical preparations of non-active ingredients, etc.
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[0212] Example - Oncogenic ErbB2 mutations in tumorigenesis
[0213] Given the importance of ErbB2 in human cancers, we systematically surveyed human cancers and identified the transmembrane (TM) and juxtamembrane (JM) domains of ErbB2, as well as recurrent somatic mutations, and these mutations have also been shown to be transformative. Furthermore, we evaluated targeted therapies in ErbB2 mutant-driven cell-based and animal cancer models and showed that they effectively blocked ErbB2 mutant-driven carcinogenesis.
[0214] Materials and methods
[0215] Tumor DNA, mutation identification
[0216] Tumor DNA mutations were identified based on the observed mutation frequency of ErbB2 mutations in tumors from patients and / or those in the TM / JM domain region and junction segment that were close to the observed mutations, as indicated (Table 1).
[0217] cell line
[0218] The primary IL-3-dependent mouse B cell line BaF3 was purchased from ATCC (American Type Culture Colle...
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