Fluorescent sericin metal nano-cluster and preparation method and application thereof

A technology of gold nanoclusters and fluorescent filaments, which is applied in nanotechnology, nanotechnology, fluorescence/phosphorescence, etc., can solve the problems of insufficient understanding of sericin, environmental pollution, waste, etc., and achieve a green preparation method, no hysteresis effect, and production The effect of high yield

Pending Publication Date: 2019-12-10
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For a long time, due to people's lack of understanding of sericin, it has been wasted in

Method used

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  • Fluorescent sericin metal nano-cluster and preparation method and application thereof
  • Fluorescent sericin metal nano-cluster and preparation method and application thereof
  • Fluorescent sericin metal nano-cluster and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047]Example 1: Preparation of Sericin-AuNCs

[0048] The preparation method is:

[0049] (1) 250 mg of sericin was added into 5 mL of pure water to prepare a 50 mg / mL sericin solution.

[0050] (2) HAuCl 4 Add water to make 25mM HAuCl 4 solution.

[0051] (3) Take 5mL sericin solution and place it in EP tube, add 1mL HAuCl to it 4 Solution, vortex mixed thoroughly for 5min.

[0052] (4) Add 1M NaOH to the mixed solution in step (3) to adjust the pH to 9, and mix again with a vortex for 5 minutes.

[0053] (5) Microwave reaction is carried out to the sample prepared in step (4), and the microwave power is 700W. There is a certain time interval between two microwaves. In this embodiment, a single microwave is used for 15s, and the next cycle of microwave treatment is carried out for 15s after an interval of 20s. A total of 12 cycles are carried out, that is, the total pure microwave reaction time is 3min, and the polymer of gold nanoclusters is obtained, which is centri...

Embodiment 2

[0054] Embodiment 2: Preparation of Sericin-AuNCs

[0055] The preparation method is:

[0056] (1) 125 mg of sericin was added into 5 mL of pure water to prepare a 25 mg / mL sericin solution.

[0057] (2) HAuCl 4 Add water to make 25mM HAuCl 4 solution.

[0058] (3) Take 5mL sericin solution and place it in EP tube, add 1mL HAuCl to it 4 Solution, vortex mixed thoroughly for 5min.

[0059] (4) Add 1M NaOH to the solution mixed in step (3) to adjust the pH to 10, the solution turns from light yellow to brown, and vortex to mix again for 10 min.

[0060] (5) Microwave reaction is carried out to the sample prepared in step (4), and the microwave power is 500W. There is a certain time interval between two microwaves. In this embodiment, a single microwave is used for 15s, and the next cycle of microwave treatment is carried out for 15s after an interval of 20s. A total of 60 cycles are performed, that is, the total pure microwave reaction time is 15min, and the polymer of go...

Embodiment 3

[0061] Example 3: Preparation of Sericin-AuNCs

[0062] The preparation method is:

[0063] (1) 250 mg of sericin was added into 5 mL of pure water to prepare a 50 mg / mL sericin solution.

[0064] (2) HAuCl 4 Add water to make 50mM HAuCl 4 solution.

[0065] (3) Take 5mL sericin solution and place it in EP tube, add 1mL HAuCl to it 4 Solution, vortex mixed thoroughly for 5min.

[0066] (4) Add 1M NaOH to the mixed solution in step (3) to adjust the pH to 11, and vortex to mix again for 5 minutes.

[0067] (5) Microwave reaction is carried out to the sample prepared in step (4), and the microwave power is 600W. There is a certain time interval between two microwaves. In this embodiment, a single microwave is used for 15 s, and the next cycle of microwave treatment is performed for 15 s after an interval of 20 s. A total of 40 cycles are performed, that is, the total pure microwave reaction time is 10 min, and the polymer of gold nanoclusters is obtained, which is centrif...

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Abstract

The invention discloses a fluorescent sericin metal nano-cluster and a preparation method and application thereof. The metal nano-cluster synthesized by taking sericin as a template is successfully prepared through a microwave radiation method. The preparation method comprises the following steps that firstly, a sericin aqueous solution is prepared, a HAuCl 4 aqueous solution is added into the sericin aqueous solution, and the mixed solution is uniformly mixed, wherein the mass ratio of the sericin to the HAuCl 4 is 25:1-25:10; and then NaOH is added to adjust the pH value of the mixed solution, and the mixed solution is put in a microwave reactor for reaction, wherein the microwave power is 500-900 W, and the reaction time is 1-20 min. According to the sericin metal nano-cluster preparedby the preparation method, the average particle size is 2.5 nm, the maximum excitation wavelength is 360 nm, the maximum emission wavelength is 480 nm, the stability is high, the biocompatibility is good, the light stability is high, the fluorescence is strong, the sensitivity is high, and the nano-cluster can be used as a probe to detect the content of Fe <3+> ions in a water environment or serumand can be served as a fluorescent probe to be applied to biological imaging research.

Description

technical field [0001] The invention belongs to the field of preparation and application of functional nanometer fluorescent materials, in particular to a fluorescent sericin gold nanocluster and its preparation method and application. Background technique [0002] In recent years, the special importance of transition ions has attracted more and more attention because some trace amounts of heavy metal ions are beneficial to human health. As an important trace element, iron plays a vital role in the human body for all organisms, and iron deficiency and excess will cause serious problems. In recent years, there has been great interest in the development of selective techniques for the quantitative determination of iron ions in biological, clinical, and environmental samples. Currently Fe 3+ Commonly used detection methods are flame atomic absorption spectrometry, graphite furnace atomic absorption spectrometry, spectrophotometric flow injection analysis, energy dispersive X-...

Claims

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Application Information

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IPC IPC(8): B22F1/00B22F9/24G01N21/64B82Y30/00B82Y40/00
CPCB22F9/24G01N21/6428G01N21/6458B82Y30/00B82Y40/00G01N2021/6432B22F1/0553B22F1/054B22F1/07B22F1/102
Inventor 王宝娟常欣高鹏吴爽李全发鲁山陈杰熊声麒吴雨芬马晓燕
Owner ANHUI NORMAL UNIV
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