A method for detecting oligosaccharides in Morinda officinalis formula granules
A technology of formula particles and detection methods, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost and low detection efficiency, and achieve the effect of rapid quality evaluation
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Embodiment 1
[0035] A method for detecting oligosaccharides in Morinda officinalis formula granules, comprising:
[0036] S11. Take 0.13g of Morinda officinalis formula granules, add 50mL of 3% methanol after grinding, weigh, extract by ultrasonic or heating under reflux for 10~45min, take it out, weigh again, supplement the reduced weight with solvent of the same concentration, and shake well , filtered, and the filtrate was obtained to obtain the test solution;
[0037] S12. Take Ness sugar standard substance, add 3% methanol to make 100 μg / ml reference substance solution;
[0038] S13. Take 1 or 3 μL of the reference substance solution and 1 μL of the test solution, and inject them into the liquid chromatograph respectively, and obtain the content of nasi sugar in the test solution by using the external standard two-point method;
[0039]S14. Using Nessose as an internal reference, simultaneously measure the content of kestose, sucrose, and Morinda officinalis oligosaccharide 5-glycan;...
Embodiment 2
[0042] A method for detecting oligosaccharides in Morinda officinalis formula granules, comprising:
[0043] S11. Take 0.13g of Morinda officinalis formula granules, add 50mL of 10% methanol after grinding, weigh, heat and reflux for extraction for 30min, take it out, weigh again, supplement the reduced weight with solvent of the same concentration, shake well, filter, take Filtrate promptly obtains need testing solution;
[0044] S12. Get Ness sugar standard substance, add 10% methanol to make 100 μg / ml reference substance solution;
[0045] S13. Take 1 or 3 μL of the reference substance solution and 1 μL of the test solution, and inject them into the liquid chromatograph respectively, and obtain the content of nasi sugar in the test solution by using the external standard two-point method;
[0046] S14. Using Nessose as an internal reference, simultaneously measure the content of kestose, sucrose, and Morinda officinalis oligosaccharide 5-glycan; the relative correction fac...
Embodiment 3
[0048] A method for detecting oligosaccharides in Morinda officinalis formula granules, comprising:
[0049] S11. Take 0.13g of Morinda officinalis formula granules, add 50mL of 5% methanol after grinding, weigh, extract by ultrasonic for 30min, take it out, weigh again, supplement the reduced weight with solvent of the same concentration, shake well, filter, and take the filtrate , to obtain the test solution;
[0050] S12. Get Ness sugar standard substance, add 5% methanol to make 100 μg / ml reference substance solution;
[0051] S13. Take 1 or 3 μL of the reference substance solution and 1 μL of the test solution, and inject them into the liquid chromatograph respectively, and obtain the content of nasi sugar in the test solution by using the external standard two-point method;
[0052] S14. Using Nessose as an internal reference, simultaneously measure the content of kestose, sucrose, and Morinda officinalis oligosaccharide 5-glycan; the relative correction factor of the s...
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