Method for detecting oligosaccharide in morinda officinalis formula granules
A technology of formula particles and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost and low detection efficiency, and achieve the effect of rapid quality evaluation and improvement of detection efficiency.
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Embodiment 1
[0035] A method for detecting oligosaccharides in Morinda officinalis formula particles, including:
[0036] S11. Take 0.13g of Morinda officinalis formula granules, add 50mL of 3% methanol after grinding, and after weighing, extract with ultrasound or heating under reflux for 10~45min, take it out, weigh again, replenish the reduced weight with the same concentration of solvent, shake well , Filter, take the filtrate, and get the test solution;
[0037] S12. Take the standard product of Nice sugar and add 3% methanol to make a 100μg / ml reference solution;
[0038] S13. Take 1 or 3μL of the reference solution and 1μL of the test solution and inject them into the liquid chromatograph respectively, and use the external standard two-point method to obtain the content of Nastose in the test solution;
[0039] S14. Using Nassi sugar as an internal reference, simultaneously determine the content of sucrose, sucrose, and Morinda officinalis 5 glycans; the relative correction factor of said s...
Embodiment 2
[0042] A method for detecting oligosaccharides in Morinda officinalis formula particles, including:
[0043] S11. Take 0.13 g of Morinda officinalis formula granules, add 50 mL of 10% methanol after grinding, weigh, heat and reflux for extraction for 30 minutes, take out, weigh again, replenish the reduced weight with the same concentration of solvent, shake well, filter, and take The filtrate is the test solution;
[0044] S12. Take the standard product of Nice sugar and add 10% methanol to make a 100μg / ml reference solution;
[0045] S13. Take 1 or 3μL of the reference solution and 1μL of the test solution and inject them into the liquid chromatograph respectively, and use the external standard two-point method to obtain the content of Nastose in the test solution;
[0046] S14. Using Nassiose as the internal reference, simultaneously determine the content of sucrose, sucrose, and Morinda officinalis oligosaccharides; the relative correction factor of said sucrose relative to Nasisi...
Embodiment 3
[0048] A method for detecting oligosaccharides in Morinda officinalis formula particles, including:
[0049] S11. Take 0.13g of Morinda officinalis formula granules, add 50mL of 5% methanol after grinding, weigh, ultrasonically extract for 30min, take it out, weigh it again, replenish the reduced weight with the same concentration of solvent, shake well, filter, and take the filtrate , The test solution is obtained;
[0050] S12. Take the standard product of Nice sugar and add 5% methanol to make a 100μg / ml reference solution;
[0051] S13. Take 1 or 3μL of the reference solution and 1μL of the test solution and inject them into the liquid chromatograph respectively, and use the external standard two-point method to obtain the content of Nastose in the test solution;
[0052] S14. Using Nassiose as an internal reference, simultaneously determine the content of sucrose, sucrose, and Morinda officinalis 5 glycans; the relative correction factor of said sucrose to Nasisi is 0.996, and th...
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