Immobilized thermophilic microflora for treating municipal sludge as well as preparation and use methods thereof
A technology for thermophilic microorganisms and municipal sludge, applied in the field of immobilized thermophilic microbial flora, can solve the problems of environmental safety hazards, reduce sludge water content, social impact, etc., achieve high mechanical strength, strong tolerance, Not easy to lose effect
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Embodiment 1
[0029] Streptococcus thermophilus, Bacillus coagulans and Candida utilis were activated and enriched to the logarithmic phase of growth, and the culture medium OD 600 was 2.5, and the bacterial fluids of Streptococcus thermophilus, Bacillus coagulans and Candida utilis were obtained respectively. Detect the bacterial counts of Streptococcus thermophilus, Bacillus coagulans and Candida utilis, and mix them according to the percentage of the bacterial counts of each strain to the total bacterial count of thermophilic microorganisms. Bacillus 15%, Bacillus subtilis 15%, Cellulomonas lutea 23% and Candida utilis 27%.
[0030] Put 40-mesh sawdust into 15% sodium sulfite solution at a ratio of 60% by weight, adjust the pH to 4.0, soak at 60°C for 48 h, and rinse with water. 20% NaOH to neutralize, wash with water, and dry. Mix sawdust with Streptococcus thermophilus, Bacillus coagulans and Candida utilis in a weight ratio of 100:40, expose at room temperature for 90 h, and dry at 40...
Embodiment 2
[0033] Streptococcus thermophilus, Bacillus coagulans, Cellulomonas xanthogenes and Candida utilis were activated and enriched to the logarithmic phase of growth, and the culture medium OD 600 was 2.3, and the bacterial liquids of Streptococcus thermophilus, Bacillus coagulans, Cellulomonas chrysogenum and Candida utilis were obtained respectively. Detect the bacterial counts of Streptococcus thermophilus, Bacillus coagulans, Cellulomonas chrysogenum and Candida utilis, and mix them according to the percentage of the bacterial counts of each strain to the total bacterial count of the thermophilic microbial flora. Streptococcus 25%, Bacillus coagulans 20%, Cellulomonas lutea 25%, and Candida utilis 30%.
[0034] Mix zeolite and sepiolite with Streptococcus thermophilus, Bacillus coagulans, Cellulomonas chrysogenum and Candida utilis in a weight ratio of 100:43, wherein the mass of zeolite and sepiolite The ratio is 1:1. Expose at room temperature for 92 hours, and dry at 43ºC...
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