Method for measuring content of in vivo biotin of insect

A method of determination, biotin technology, applied in the field of determination, can solve the problems of small insect physiological science research, small detection limit range, long detection time, etc., and achieve the effects of low cost, improved efficiency, and simple cultivation method
CN110618281AActive Publication Date: 2019-12-27SHENYANG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENYANG AGRI UNIV
Publication Date
2019-12-27

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Abstract

The invention relates to a method for measuring the content of in vivo biotin of an insect. The method comprises the following steps: (1) extracting an insect sample; (2) preparing a biotin gradient solution; (3) preparing a biotin assay culture solution; and (4) determining biotin. The lactobacillus plantarum, various culture media and the like used in the detection method are common strains or consumables in the market, the cost is low, the biotin detection cost is remarkably reduced, and the culture method is simple; the detection time of the method is 22 hours and is reduced by 22 to 26 hours compared with that of a commercial kit, and the efficiency of insect vitamin detection is improved.
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Description

technical field

[0001] The invention relates to a method for measuring, in particular to a method for measuring biotin content in insects. Background technique

[0002] B vitamins are an indispensable nutrient element for animals. B vitamins are coenzymes for many enzymes in animals. Biotin (vitamin B7), is the coenzyme of acetyl-CoA. Soluble in water, stable to acid, easily destroyed by alkali. It has a vital impact on the metabolic process of living organisms. Quantitative detection of biotin in organisms is the basis for the study of life metabolism. At present, the detection methods mainly include microbial method, fluorescence analysis, spectrophotometry, electrochemical method, high performance liquid chromatography or high performance capillary electrophoresis and so on. However, these methods are commonly used in food and feed biotin detection.

[0003] There are few studies on the determination of biotin in insects. Only bedbugs have used high performance liqu...

Claims

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