Morchella esculenta mother strain culture medium and morchella esculenta mother strain preparation method
The technology of morel and culture medium is applied in the field of the culture medium of mother hickory chick and the preparation field of hickory hickory mother species, which can solve the inconvenience, the inability to store potatoes for a long time, and the mycelium growth speed of hickory chick mother species Slow and other problems, to achieve the effect of low cost, short full tube time, and convenient source
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0022] Preparation of morel mother species:
[0023] (1) Juicing: Weigh each substance according to the mass percentage, take the potato, peel and remove the bud eyes, cut into 1m 3 Small pieces, add small potato pieces into water, boil on high heat, then switch to low heat and continue to boil for about 20 minutes, filter to get juice;
[0024] (2) Culture medium preparation: make up the lost water in the filtered juice, add agar, simmer to dissolve, then add glucose, stir until dissolved, and the pH is natural;
[0025] (3) Subpackage: Subpackage the culture medium in the Erlenmeyer flask, the subpackage of the culture medium is 2 / 3 of the capacity of the Erlenmeyer flask, seal it with a silica gel plug, and wrap the bottle mouth with kraft paper;
[0026] (4) Sterilization: subject the wrapped triangular flask and packaged clean petri dish to high-pressure steam sterilization, and sterilize at 121° C. for 30 minutes;
[0027] (5) Pour plate: under aseptic conditions, pour...
Embodiment 1
[0100] Culture medium: 2% glucose, 5% rice, 2% agar, 0.1% calcium sulfate, the balance is water, and the pH value is natural.
[0101] Preparation of morel mother species:
[0102] (1) Juicing: put 50g of rice into a small pot, add 900mL of water, bring to a boil on high heat, then switch to a slow fire and continue to boil for 20 minutes, filter to get the juice;
[0103] (2) Culture medium preparation: add 20g of agar to the filtrate to make up for the water loss, simmer to dissolve, then add 20g of glucose and 1g of calcium sulfate, stir until dissolved, set the volume to 1L, and the pH is natural;
[0104] (3) Subpackage: subpackage the medium into test tubes, the subpackage of the medium is 1 / 5 of the length of the test tube, and seal it with a silica gel plug;
[0105] (4) Bundling: get 7 test tubes with culture medium and bundle them together, and wrap the test tube mouth with double-layer newspaper;
[0106] (5) Sterilization: subject the bundled test tubes to high-p...
Embodiment 2
[0112] Medium: 2% glucose, 5% rice, 2% agar, 0.1% calcium sulfate, the balance is water, pH 8.
[0113] Preparation of morel mother species:
[0114] (1) Juicing: Put 50g of rice into a small pot, add 900mL of water, bring to a boil on high heat, then switch to a slow fire and continue to boil for 15 minutes, filter to get the juice;
[0115] (2) Culture medium preparation: add 20g of agar to the filtrate to make up for water loss, simmer to dissolve, then add 20g of glucose and 1g of calcium sulfate, stir until dissolved, set the volume to 1L, and adjust the pH to 8;
[0116] (3) Subpackage: subpackage the medium into test tubes, the subpackage of the medium is 1 / 4 of the length of the test tube, and seal it with a silica gel plug;
[0117] (4) Bundling: Get 7 test tubes with culture medium and bundle them together, and wrap the test tube mouth with kraft paper;
[0118] (5) Sterilization: subject the bundled test tubes to high-pressure steam sterilization, and sterilize at...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com