Dual fluorescent PCR primer and kit for identifying ASFV strain and CD2v gene-deleted strain

A gene deletion and dual fluorescence technology, applied in the direction of microorganism-based methods, DNA/RNA fragments, microorganisms, etc., can solve the problems that wild strains are difficult to identify and cannot be identified
CN110628956AInactive Publication Date: 2019-12-31青岛立见生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
青岛立见生物科技有限公司
Publication Date
2019-12-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to double fluorescent PCR primers and a kit for identifying ASFV strain and CD2v gene-deleted strains, and belongs to the technical field of biology. The sequences of the primersare shown as SEQ ID NO.1-6. The invention also discloses the kit comprising the primers. The kit is composed of the following components: a fluorescent PCR reaction solution, a mixed solution containing the primers, a positive control and a negative control. The six primers are all placed in one tube, are mixed together for use, do not interfere with one another, do not influence subsequent PCR amplification and fluorescence signal value acquisition, and can identify African swine fever virus wild strain and gene-deleted strains.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a detection kit for identifying and detecting African swine fever virus wild strains and gene deletion strains. Background technique

[0002] African swine fever virus (ASFV) is the only member of the African swine fever virus family. Its genome is a single-stranded DNA virus with a size of 150-190kb and can encode at least more than 150 proteins. ASFV can infect domestic pigs and wild boars at various stages, and the mortality rate is as high as 100%. ASFV can be transmitted through direct and indirect contact, and ASFV-contaminated pork, cured ham, swill, feed, means of transportation, and personnel can all become sources of infection. Since the African swine fever epidemic was first discovered in 1921, the African swine fever virus has spread from Africa to Europe, Asia and other places through various means such as airplanes and ships. In 2018, African swine fever also broke ou...

Claims

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