The invention relates to the technical field of dairy leavening agents, and discloses a preparation method of a direct vat set yoghurt
leavening agent with high
fermentation activity, and the preparation method comprises the following steps: S1, scribing a
fermentation strain in a flat plate for rejuvenation, screening a single colony, and preserving a preferred mother strain; s2, inoculating the preferable mother strain into a
liquid culture medium, activating for two generations, then transferring into skim milk
powder fermentation liquor, and culturing by using an acid-base neutralizing agent; s3, after fermentation is finished, adding a
polysaccharide protection buffer agent, and uniformly mixing the
polysaccharide protection buffer agent with the fermentation liquid under a low-temperature condition; s4, after the thalli are enriched through disc
centrifugation, vacuum
freeze drying is conducted, and the direct vat set yoghurt
leavening agent is prepared. According to the method, two generations of screening are carried out on the activity of the zymophyte mother strain, then the fermentation activity and the stability of characters are guaranteed, a
polysaccharide protection buffer agent is added into the fermentation liquor obtained after fermentation of the optimized mother strain, then disc type centrifugal treatment is adopted, mechanical damage of
centrifugation to thalli is reduced, and the effective
viable count and the fermentation activity in zymophyte
powder are improved.