Composition and use thereof in protein extraction and/or dissolution
A protein extraction and composition technology, applied in the field of extraction and/or dissolution, can solve the problems of accurate understanding and in-depth understanding of the outer membrane of Geobacter sulfur-reducing, inactivation of catalytic subunits, etc., to avoid protein aggregation , easy to operate, avoid the effect of deactivation
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[0033] Example 1, Extraction and Characterization of Cytochrome c Components in the Outer Membrane of Geobacter Sulfurreducens Under Anaerobic Conditions
[0034] The mineral salt medium formula used in this example is as follows: 20mM sodium acetate, 40mM fumaric acid, 1.50g / L ammonium chloride, 0.60g / L disodium hydrogen phosphate, 0.10g / L potassium chloride, 2.50g / L carbonic acid Sodium hydrogen and 1% trace element solution. Trace element solution includes 1.50g / L nitrilotriacetic acid, 3.00g / L magnesium sulfate heptahydrate, 0.50g / L manganese sulfate hydrate, 1.00g / L sodium chloride, 0.10g / L ferrous sulfate heptahydrate, 0.18g / L cobalt sulfate heptahydrate, 0.10g / L calcium chloride dihydrate, 0.18g / L zinc sulfate heptahydrate, 0.01g / L copper sulfate pentahydrate, 0.02g / L potassium aluminum sulfate dodecahydrate, 0.01g / L boric acid , 0.01g / L sodium molybdate dihydrate, 0.03g / L nickel chloride hexahydrate, 0.30mg / L sodium selenite pentahydrate, 0.40mg / L sodium tungstate di...
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