Detection method of

A technology for the detection of metystrobin and its metabolites, which is applied in the field of detection of the residues of metystrobin and its metabolites in tobacco, and can solve the problem of instability, inability to directly measure metystrobin and its metabolites, cumbersome and time-consuming methods, etc. time-saving, simple sample preparation, high selectivity and sensitivity

Active Publication Date: 2019-12-31
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology

[0003] Metystrobin (2,4-DNOPC) is unstable and easily photolyzed or hydrolyzed into the corresponding metabolite (2,4-DNOP). At present, the methods reported for the detection of metystrobin residues mainly include high performance liquid chromatography , liquid chromatography-mass spectrometry, these methods can no

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Embodiment 1

[0047] 1. Instruments and reagents: EVOQ GC-TQ gas chromatography-tandem mass spectrometer (Bruker, USA), Millipore ultra-pure water machine (Millipore Simplicity, USA), Eppendorf 5804 high-speed centrifuge (Eppendorf, Germany), Talboys digital multi-type Tubular vortex mixer (Shanghai Anpu Scientific Instrument Co., Ltd.). Methanol, acetonitrile, HPLC grade, American Fisher Company. Medistrobin (2,4-DNOPC) standard (purity 93%, provided by Dr, Germany), mediclostrobin metabolite (2,4-DNOP) standard (purity 98.5%, provided by Dr, Germany) ).

[0048] 2. Detection method for the residual amount of metystrobin in tobacco:

[0049] 1) Preparation of standard solution: Accurately weigh the standard substance (accurate to 0.1 mg) of medistrobin and medistrobin metabolite (medistrobin phenol), dissolve in methanol and dilute to 100mL brown volumetric flask, Prepare a single standard stock solution of 1 mg / L and store it in a refrigerator at -20°C in the dark for making a standard...

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Abstract

The invention discloses a method for detecting residual quantity of meptyldinocap and metabolites thereof in tobacco. A tobacco sample is detected by adopting acetonitrile ultrasonic extraction and gas chromatography-negative chemistry source tandem mass spectrometry. The detection method disclosed by the invention is simple and fast in sample pre-treatment, the sensitivity, the accuracy and the precision meet the detection requirements of pesticide residue, and the method is suitable for simultaneously detecting the meptyldinocap and metabolites thereof in the tobacco.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue detection, and in particular relates to a method for detecting residues of metystrobin and its metabolites in tobacco. Background technique [0002] Metrastrobin is a new type of powdery mildew fungicide, which has good control effect on powdery mildew on pome fruit, cucurbit crops, stone fruits, citrus, tobacco, vegetables and ornamental plants. Some herbivorous mites also have a certain inhibitory effect. Metrastrobin is a single isomer isolated from six isomers of dinocap (CAS: 39300-45-3). Due to its outstanding control effect, the drug has been used in Europe, the United States and other countries for many years , registered and used in China in 2018. Internationally, the maximum residue limit (MRL) requirements for metrastrobin are very strict, and the detection of its residues is very important. At present, the European Union has established the maximum residue limit (MRL, the ...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/72G01N2030/143
Inventor 师君丽孔光辉李勇逄涛吴玉萍
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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