Terahertz metamaterial biosensor and its preparation method and detection method

A technology of biosensors and metamaterials, applied in the fields of analyzing materials, analyzing materials through optical means, instruments, etc., can solve problems such as time-consuming, complicated operation, and inability to meet rapid detection

Active Publication Date: 2020-10-20
INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Early clinical diagnostic methods for a certain protein concentration in serum samples mainly used some biochemical methods, such as enzyme-linked reaction (ELISA), Western blot (WB), immunofluorescence (IFA), etc. However, these methods are complicated to operate and It takes a long time and cannot meet the current needs of rapid detection

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  • Terahertz metamaterial biosensor and its preparation method and detection method
  • Terahertz metamaterial biosensor and its preparation method and detection method
  • Terahertz metamaterial biosensor and its preparation method and detection method

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preparation example Construction

[0084] The metal preparation method is as follows: the growth method of the bottom metal layer is electroplating or magnetron sputtering, and the growth method of the surface layer gold is electron beam evaporation.

[0085] In some embodiments of the present invention, the response waveband of the biosensor is in the terahertz range, and the waveband range is 0.5-3THz.

[0086] In some embodiments of the present invention, the antibodies or probes modified on the metamaterial to recognize different cancer markers are: CEA (carcinoembryonic antigen), CA125 (glycoprotein antigen 125), AFP (alpha-fetoprotein) , CA15-3 (glycoprotein antigen 15-3), GGT-II (glutamyl transpeptidase isozyme II), CA19-9 (glycoprotein antigen 19-9), CA42 (glycoprotein antigen 42), microRNA4484 , microRNA3646 and other conventional cancer marker antibodies and microRNA probes.

[0087] As mentioned above, the detection method of the negative refractive index terahertz metamaterial biosensor for early w...

Embodiment 1

[0133] The negative refractive index terahertz metamaterial biosensor was prepared according to the following steps:

[0134] (1) Substrate preparation: Boil in acetone solution for 8 minutes to remove organic pollutants on the substrate surface before performing metamaterial processing, then boil in absolute ethanol solution for 10 minutes to remove acetone, and finally rinse with deionized water for three Blow dry with nitrogen.

[0135] (2) Metamaterial structure construction:

[0136] The transfer of the metamaterial pattern on the substrate is realized by micro-nano-fabrication lithography technology. First, a photoresist (AR-N4340) was coated on the previously processed substrate by spin coating. Prebake at 95°C for 15 minutes, exposure to build those metamaterial structures on the substrate, and postbake at 105°C for 10 minutes.

[0137] The prepared metamaterial structure is a rotating double ring structure, the metamaterial pattern is a rotating double split ring, ...

Embodiment 2

[0146] This embodiment is basically the same as Embodiment 1, the difference is:

[0147] In step (4), the substrate is a 300 μm silicon substrate, which is thinned to 150 μm by a substrate thinning process. After the thinning process, it should be boiled in acetone solution for 10 minutes to remove organic pollutants on the substrate surface, then boiled in absolute ethanol solution for 15 minutes to remove acetone, finally rinsed with deionized water three times, and dried with nitrogen.

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Abstract

The invention discloses a terahertz metamaterial biosensor and a preparation method and detection method thereof. The biosensor comprises a substrate, a metamaterial structure, and identification units. The metamaterial structure arranged on the substrate is used for responding to terahertz waves; and a plurality of detection areas are arranged on the metamaterial structure. The identification units modified on the detection areas are used for identifying different markers. According to the terahertz metamaterial biosensor, the adopted metamaterial has an asymmetric high-Q-value structure andis sensitive to the change response of surrounding environment media highly; the cancer marker is combined with the surface of the metamaterial structure to cause the red shift of a terahertz transmission spectrum; and the concentration of the cancer marker and peak position shift are in functional correlation. The concentration of the marker in the serum of a patient can be detected by a comparison curve of the detection concentration of the marker and the terahertz transmission spectrum.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a terahertz metamaterial biosensor, a preparation method and a detection method thereof. Background technique [0002] In the early stage of cancer development, there are often no obvious disease signals, which are often ignored by patients. When there are symptoms, the cancer has a high degree of development or has metastasized. Studies have shown that cancer cells will produce different cancer marker proteins after formation, which can help people diagnose whether there are corresponding cancer cells in the body. Therefore, cancer marker protein concentrations play a key role in early cancer diagnosis. [0003] Terahertz spectroscopy has the advantages of immediacy, label-free and non-destructive detection. With the development of micro-nano processing technology, terahertz sensors based on metamaterials have received great attention. By changing the structure and size of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N21/3581G01N33/536G01N33/574
CPCG01N21/3581G01N33/531G01N33/536G01N33/574
Inventor 崔宁关敏徐梦轲张杨曾一平
Owner INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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