Epitope polyclonal antibody of toxoplasma gondii Actin protein and application of epitope polyclonal antibody

Abstract

The invention discloses an epitope polyclonal antibody of toxoplasma gondii Actin protein and an application of the epitope polyclonal antibody. According to the epitope polyclonal antibody of toxoplasma gondii Actin protein, C terminal 227-237 in the amino acid sequence of toxoplasma gondii Actin is used as an antigenic determinant peptide fragment being good in specificity, toxoplasma gondii antigenic determinant polypeptide is obtained, and the epitope polyclonal antibody of the toxoplasma gondii Actin protein is further prepared. The designed polypeptide is simple in epitope components, induced immune reaction is high in applicability, and the toxoplasma gondii Actin protein can be specially detected and distinguished. The epitope polyclonal antibody is mainly used for detecting the expression level respect of the toxoplasma gondii protein as an internal reference contrast, can accurately determine the relative expression level of target protein, can position toxoplasma gondii invading cells through immunofluorescence, and provides great help for further research of a molecular action mechanism.

Description

technical field [0001] The invention relates to an epitope polyclonal antibody of the Toxoplasma gondii Actin protein and application thereof, belonging to the field of immunology. Background technique [0002] Toxoplasma gondii is a kind of intracellular parasitic protozoa which is widely prevalent among humans and animals, and its infection seriously endangers human health and the development of animal husbandry. Existing research results show that multiple molecules such as rhomboids (ROMs), actin (Actin) and micronemeproteins (MICs) of Toxoplasma gondii are involved in the invasion process. [0003] Actin is an important skeleton protein, which can be roughly divided into six types. Among them, β-Actin is widely distributed in various tissues, with a size between 42kDa-43kDa, abundant and relatively constant expression, and is commonly used in PCR. Internal reference, its antibody is a good internal reference index for Western Blot. [0004] Unfortunately, because Acti...

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Problems solved by technology

[0004] Unfortunately, because Actin is highly conserved among different species, it is difficult to obtain a better antiserum for specific species of Actin. So far, there is no commercial Actin antibody for Toxoplasma gondii, which is lacking in detecting the expression level of Toxoplasma gondii protein. The internal control can not accurately determine the relative expression of the target protein, which limits further research on its molecular mechanism of action

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