Composite mycorrhiza biological fertilizer, and preparation method and application thereof

Abstract

The invention belongs to the technical field of microbial fertilizers and preparations, and discloses a composite mycorrhiza biological fertilizer, and a preparation method and an application thereof.The preparation method comprises the following steps: activating a preserved strain with a solid activation medium, picking single colonies, culturing the single colonies on a same medium plate according to pairwise crossed three-zone lines of member strains in groups at 30 DEG C for 3 days, and repeating the culture for 3 times; inoculating antagonistic bacteria-free strains and mycorrhizal fungi into respective liquid culture media; preparing 100 ml of a solution from sodium alginate with the concentration of 3% and polyvinyl alcohol with the concentration of 3% at 35 DEG C; adding 0.5% biochar, preparing 100 ml of a solution at 35 DEG C, sterilizing the solution at 121 DEG C for 20 min, cooling the sterilized solution to 35 DEG C, taking 2 g of composite functional bacteria and 2 g ofmycorrhizal fungi thalli, adding the taken substances into an embedding medium solution, and performing uniform mixing in a constant-temperature magnetic stirrer. The functional bacterial system and mycorrhizal fungus mixing embedding immobilization research and the embedding immobilized particle promote Chinese pine seedlings.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers and preparations, and in particular relates to a compound mycorrhizal biological fertilizer and its preparation method and application. Background technique [0002] At present, the closest existing technology: Academician Chen Huagui, the founder of soil microbiology in China, defines microbial fertilizers as a type of "specific products containing live microorganisms, and the fertilizer effect obtained by plants mainly depends on the life activities of living microorganisms in the products. Microorganisms Fertilizers are used in agricultural and forestry production. After being applied to the soil, there are many types of growth-promoting bacteria or bio-control bacteria that stably colonize the root soil of plants to form a specific microbial preparation of dominant flora. It has specific fertilizers such as phosphorus, potassium, nitrogen fixation and pest control. Effect, and in...

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Problems solved by technology

[0011] (2) Some enterprises in the field of micro-fertilizers are slow to update equipment, have primitive production technology, and poor product quality

In addition, the application effect of micro-fertilizers is unstable, which is affected by many factors such as application methods and environmental conditions, which hinders the further promotion and application of the micro-fertilizer industry

[0013] (3) PGPR cannot be stably colonized in micro-fertilizers

At present, most enterprises are still using Bacillus-based nitrogen-fixing, phosphorus- and potassium-solubilizing bacteria, but fungal functional bacteria such as VA and ECM are rarely used.

[0015] (4) Research on the mechanism of action of micro-fertilizer products is not deep enough

[0016] Studies have shown that the mechanism of PGPR promoting plant growth in micro-fertilizers is divided into direct mechanism and indirect mechanism. The difference between the two mechanisms is mainly based on the location of the mechanism. The PGPR effect occurs outside the plant as an indirect mechanism, and the PGPR effect occurs inside the plant as a direct mechanism. Mechanism, the direct mechanism of PGPR to promote plant growth is still in the study of biological nitrogen fixation, phosphorus and potassium, plant hormone production indole-3-acetic acid (IAA), cytokinin (CK), gibberellins (GAs), etc. The indirect mechanism research is limited to siderophore production, lyase production, antibiotic production, induction of plant systemic resistance and ACCD, etc., while the interaction mechanism between bacteria and bacteria and the interaction between

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