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One for detecting cu 2+ The ratiometric fluorescent sensor and its preparation method and application

A ratiometric fluorescent sensor technology, applied in the field of fluorescent sensing, can solve the problems of time-consuming, easy to be polluted, etc., and achieve the effect of overcoming interference, low price, and wide practical value

Active Publication Date: 2021-10-15
HUBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is exactly to detect Cu at present 2+ The method requires the use of pre-separation technology, and this technology has problems such as time-consuming and easy to be polluted, providing a method for detecting Cu 2+ The ratiometric fluorescent sensor and its preparation method and application, the ratiometric fluorescent sensor CdTe QDs / GCNNs provided by the invention can easily and concisely measure Cu in tea, fruit juice and red wine samples 2+ The method has the advantages of cheap, simple, high sensitivity, good selectivity, wide linear range, short detection time, etc., and has a good application prospect

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  • One for detecting cu <sup>2+</sup> The ratiometric fluorescent sensor and its preparation method and application
  • One for detecting cu <sup>2+</sup> The ratiometric fluorescent sensor and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0034] A method for detecting Cu in this embodiment 2+ The preparation method of the ratiometric fluorescent sensor comprises the following steps:

[0035] (1) Synthesis of water-soluble cadmium antimonide quantum dots (CdTe QDs) solution

[0036] Weigh 53.3mg Cd(CH 3 COO) 2 2H 2 O was dissolved in 50.0 mL of double distilled water, and 18.0 μL of thioglycolic acid was added thereto, and NaOH solution with a concentration of 1.0 mol / L was added dropwise to adjust the pH of the solution to 10.5, and the solution was stirred for 5 min to obtain liquid A; then weighed 10.2 mg K 2 TeO 3 Dissolve in 50.0mL double distilled water to obtain liquid B; mix liquid A and liquid B, then add 80mg NaBH to the mixed solution 4 , continue to stir for 5 minutes, then heat up the mixed solution to 100 ° C, and reflux for 8 hours.

[0037] (2) Synthesis of graphitic carbon nitrides (GCNNs)

[0038] Accurately weigh 0.075g of sodium citrate dihydrate and 0.22g of melamine, dissolve them i...

Embodiment 2

[0043] A method for detecting Cu in this embodiment 2+ The ratiometric fluorescence sensor for Cu 2+ The application of quantitative detection includes the following steps:

[0044] (1) ratiometric fluorescence sensor to Cu in standard solution 2+ Content detection

[0045] Take 0, 3.0, 4.0, 5.0, 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 μL of the concentration of 1 μg / mL Cu 2+ The standard solution was placed in 14 volumetric flasks, and 705 μL of CdTe QDs / GCNNs ratiometric fluorescent sensor solution was added to each volumetric flask, then the volume was adjusted to 5.0 mL with double distilled water, and the concentration gradient was obtained after ultrasonication for 5 min until the dispersion was uniform. Blank, 0.6ng / mL, 0.8ng / mL, 1.0ng / mL, 2.0ng / mL, 4.0ng / mL, 6.0ng / mL, 8.0ng / mL, 10.0ng / mL, 12.0ng / mL, 14.0ng / mL mL, 16.0ng / mL, 18.0ng / mL and 20.0ng / mL of Cu 2+ Standard solution, under the excitation wavelength of 340nm, detect the fluorescence spectrum of all stand...

Embodiment 3

[0053] In order to further verify the ratiometric fluorescence sensor of the present invention to Cu 2+ To determine the accuracy of the content detection results, the tea sample, orange juice sample and red wine sample in Example 2 were all subjected to a standard addition recovery test, and the test results are shown in Table 2 below.

[0054] Table 2 Results of standard recovery experiment

[0055]

[0056] The results in Table 2 show that Cu 2+ The recovery rate is 100.8-107.6%, and the test result has good accuracy. Based on the above results, it is indicated that CdTe QDs / GCNNs ratiometric fluorescence sensor can be well applied to detect Cu 2+ content.

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Abstract

The invention discloses a method for detecting Cu 2+ The ratiometric fluorescent sensor and its preparation method and application, by physically mixing orange-yellow CdTe QDs and blue GCNNs to obtain pinkish-yellow GCNNs / CdTe QDs as a dual-emission ratiometric fluorescent nanosensor, the ratiometric fluorescent nanosensor for Cu 2+ Has high selectivity and sensitivity; in Cu 2+ Under the action of the sensor, when the sensor is excited at 340nm, the orange-yellow CdTe QDs fluorescence is used as the detection signal along with the Cu 2+ The concentration change showed a linear quenching relationship, while the fluorescence intensity of the blue GCNNs remained unchanged, successfully establishing the fluorescence signal I 436 / I 572 with Cu 2+ The linear relationship of the concentration; Cu can be obtained according to the color change under the ultraviolet light 2+ Content range; the present invention has the characteristics of low price, simple and intuitive, wide detection range, short detection time, etc., and has extensive practical value.

Description

technical field [0001] The invention relates to the technical field of fluorescence sensing, in particular to a method for detecting Cu 2+ The ratiometric fluorescent sensor and its preparation method and application. Background technique [0002] Copper(II) is a micronutrient and one of the most important transition metals in the human body. It plays an important role in bone formation and cellular respiration function, hemoglobin, elastin and collagen synthesis, and removal of excess free radicals and cholesterol in the body. . Copper deficiency can lead to many diseases such as anemia, neutropenia and bone abnormalities. However, excessive copper intake can lead to severe copper poisoning, such as causing lethargy and high blood pressure, and inducing Alzheimer's disease and Parkinson's disease. The National Research Council recommends that adults, children and infants consume 1.5-3.0, 1.5-2.5 and 0.4-0.6 mg per day, respectively. Therefore, a method for the detection...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/88C09K11/65B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C09K11/65C09K11/883G01N21/643G01N2021/6432
Inventor 吴一微何开礼柳恋
Owner HUBEI NORMAL UNIV