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Culture medium for rapidly proliferating neural stem cells

A neural stem cell and cell culture technology, applied in the field of fast-proliferating neural stem cell culture medium, can solve the problems affecting the proliferation rate of neural stem cells, single medium components, etc., and achieve the effects of reducing culture cost, avoiding local differentiation, and improving division speed.

Pending Publication Date: 2020-01-14
安徽科门生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the object of the present invention is to provide a culture medium for rapidly proliferating neural stem cells, which solves the problem that the medium components in the prior art are single and affects the proliferation speed of neural stem cells.

Method used

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Examples

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Embodiment 1

[0022] An embodiment of the present invention provides a culture medium for rapidly proliferating neural stem cells, which includes the following composition: DMEM / F12 medium is used as a base material, the content of epidermal growth factor is 20 ng / mL, the content of transferrin is 10 μg / mL, alkali The content of fibroblast growth factor was 20ng / mL, the content of fibronectin was 25ng / mL, the content of type IV collagen was 50ng / mL, and the content of antibiotics was 90U / mL penicillin, 0.13mg / mL Streptomycin and 300ng / mL amphotericin are mixed, the content of bovine serum albumin is 60 μg / mL, the content of non-essential amino acids is 9 μmol / mL and the volume content accounts for 2% of the DMEM / F12 medium for B27 cell culture additive.

[0023] Wherein, the non-essential amino acid is one or a mixture of glutamic acid, tyrosine, proline, glycine, cystine, alanine, aspartic acid or serine.

[0024] A method for rapidly proliferating neural stem cell culture medium, compris...

Embodiment 2

[0027] An embodiment of the present invention provides a culture medium for rapidly proliferating neural stem cells, which includes the following composition: DMEM / F12 medium is used as a base material, the content of epidermal growth factor is 15 ng / mL, the content of transferrin is 15 μg / mL, alkali The content of fibroblast growth factor was 25ng / mL, the content of fibronectin was 15ng / mL, the content of type IV collagen group was 60ng / mL, and the content of antibiotics was 95U / mL penicillin, 0.05mg / mL Streptomycin and 250ng / mL amphotericin are mixed, the content of bovine serum albumin is 40μg / mL, the content of non-essential amino acids is 8μmol / mL and the volume content accounts for 3% of the DMEM / F12 medium B27 cell culture additive.

[0028] Wherein, the non-essential amino acid is one or a mixture of glutamic acid, tyrosine, proline, glycine, cystine, alanine, aspartic acid or serine.

[0029] A method for rapidly proliferating neural stem cell culture medium, compris...

Embodiment 3

[0032] An embodiment of the present invention provides a culture medium for rapidly proliferating neural stem cells, which includes the following composition: DMEM / F12 medium is used as a base material, the content of epidermal growth factor is 25 ng / mL, the content of transferrin is 12 μg / mL, alkali The content of fibroblast growth factor was 15ng / mL, the content of fibronectin was 20ng / mL, the content of type IV collagen group was 70ng / mL, and the content of antibiotics was 110U / mL penicillin, 0.1mg / mL Streptomycin and 200ng / mL amphotericin are mixed, the content of bovine serum albumin is 50 μg / mL, the content of non-essential amino acids is 10 μmol / mL and the volume content accounts for 2% of the DMEM / F12 medium for B27 cell culture additive.

[0033] Wherein, the non-essential amino acid is one or a mixture of glutamic acid, tyrosine, proline, glycine, cystine, alanine, aspartic acid or serine.

[0034] A method for rapidly proliferating neural stem cell culture medium, ...

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PUM

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Abstract

The invention discloses a culture medium for rapidly proliferating neural stem cells. The culture medium comprises components as follows: taking a DMEM / F12 culture medium as a base material, 15-25 ng / mL of an epidermal growth factor, 10-15 [mu]g / mL of transferrin, 15-25 ng / mL of an alkaline fibroblast growth factor, 15-25 ng / mL of fibronectin, 50-70 ng / mL of a tetratype collagen group, 0.05-0.13 mg / mL of antibiotics, 40-60 [mu]g / mL of bovine serum albumin, 8-10 [mu]mol / mL of non-essential amino acid and a B27 cell culture additive. The non-essential amino acid is a nutrient substance necessaryfor neutral stem cells, the cell differentiation phenomenon caused by incomplete absorption of nutrient substances is avoided, and meanwhile, the cracking speed affected by toxin in a classificationprocess is decreased by addition of antibiotics.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a culture medium for rapidly proliferating neural stem cells. Background technique [0002] With the development of society and the improvement of technology, beauty has been constantly changing and improving from the content to the form. According to the different connotations of beauty, modern beauty can be divided into two parts: life beauty and medical beauty. Generally speaking, life beauty is to use professional skin care cosmetics and professional beauty equipment as auxiliary equipment to provide all-round care and maintenance for the facial skin of the human body. However, the maintenance period is limited and regular maintenance is required for better Maintain facial skin. There is also a series of surgical treatments on the facial skin by means of medical treatment in order to maintain the facial skin for a long time, but this method has certain surg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2501/11C12N2500/24C12N2501/115C12N2533/52C12N2533/54C12N2501/998C12N2500/32C12N2501/33C12N2500/02
Inventor 张鹏成
Owner 安徽科门生物科技有限公司
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