HPLC method for separating artabsin A from artabsin B

Abstract

The invention discloses an HPLC method for separating artabsin A from artabsin B. According to the method, a Waters Sunfire C18 liquid chromatographic column in the specification of 4.6 mm*250 mm and5 micron is adopted; the mobile phases include a phase A of a methanol aqueous solution in volume percentage concentration of 10% and with 10mM of a chiral additive and a phase B of a methanol aqueoussolution in the volume percentage concentration of 90% and with 10mM of a chiral additive; an elution process includes 0-5 min, 0% B; 5-20 min, 0%-80% of B; 20-25 min, 80%-0% of B; the flow velocityis 1.0mL/min; and the column temperature is 35 DEG C. According to the method, the chiral additive D-prolinamide is added into the mobile phase, so that the artabsin A can be separated from the artabsin B without the help of a chiral chromatographic column. Therefore, the HPLC method disclosed by the invention can be used for detecting whether the artabsin A is mixed with the artabsin B or whetherthe artabsin B is mixed with the artabsin A.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an HPLC method for separating cinnamate A and B. Background technique [0002] Enininactone A and eninininactone B are a pair of isomers, the difference is only in one connection -OH and -CH 3 The chirality of the carbon atom is different, and the chemical structural formulas of cinnamate A and B are as follows: [0003] [0004] Since the chemical structures of enigninactone A and enigninactone B are very similar, common HPLC methods are difficult to separate the two, so it cannot be used to detect whether enigninactone A is mixed with enigninactone B or enininactone Whether the lactone B is mixed with galactone A. [0005] The present invention is proposed for efficient separation of bingenolactone A and bingenicalactone B. Contents of the invention [0006] The present invention aims to overcome the deficiencies in the prior art, and provides an HPLC method for sepa...

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Problems solved by technology

[0004] Since the chemical structures of enigninactone A and enigninactone B are very similar, common HPLC methods are difficult to separate the t

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