Personalized 3D neural culture system for generating human oligodendrocytes and studying myelination in vitro

A technology of oligodendrocytes and astrocytes, applied in 3D culture, nervous system diseases, cell culture active agents, etc., can solve problems such as lack of neurons or impaired glial function

Active Publication Date: 2020-01-17
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, in the area of ​​neurotoxicity, there is still a lack of assays for human cells that can assess impaired neuronal or glial function

Method used

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  • Personalized 3D neural culture system for generating human oligodendrocytes and studying myelination in vitro
  • Personalized 3D neural culture system for generating human oligodendrocytes and studying myelination in vitro
  • Personalized 3D neural culture system for generating human oligodendrocytes and studying myelination in vitro

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Embodiment 1

[0088] Previous methods to generate human oligodendrocytes and mimic the in vivo myelination process have been performed in two-dimensional (2D) cultures. In these protocols, myelination is studied when encapsulation of oligodendrocytes is performed around synthetic nanopillars. These methods do not allow the study of myelination as it occurs as a three-dimensional process in the human brain. Alternatively, human stem cell-derived oligodendrocytes have been transplanted into rodents, but it is unclear to what extent the mouse environment affects human oligodendrocyte biology and myelination, and these methods Laborious and does not allow for high-throughput screening. The method described below is the first to generate myelinating oligodendrocytes in vitro in three-dimensional (3D) cultures derived solely from human hiPSCs or hESCs, allowing human myelination to be studied in vitro under healthy and diseased conditions.

[0089] hiPSC maintenance and aggregation. In iPSC me...

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Abstract

Human pluripotent stem cells are differentiated in vitro into oligodendro-spheroids comprising oligodendrocytes for use in analysis, screening programs, and the like.

Description

[0001] Federally funded research and development [0002] This invention was made with United States Government support under Grant No. MH107800 awarded by the National Institutes of Health. The government has certain rights in this invention. Background technique [0003] Progress in understanding the complex development of the human central nervous system and in elucidating the mechanisms underlying neurological and psychiatric disorders in patients has been greatly limited by limited access to functional human brain tissue. Although studies in rodents and other mammals have provided important insights into the fundamentals of neurodevelopment, little is known about the cellular and molecular processes responsible for the massive expansion of the primate forebrain, many of which are specific to humans. Characteristics are also poorly understood. In recent years, a paradigm shift has been achieved in the field with the introduction of cellular reprogramming, during which te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/30A61K35/545A61P25/00C12N5/00
CPCC12N2500/38C12N2501/105C12N2501/12C12N2501/13C12N2501/135C12N2501/33C12N2501/385C12N2501/395C12N2501/415C12N2501/727C12N2513/00C12N2533/52C12N2501/999C12N5/0622G01N33/5058C12N5/0696G01N33/5032C12N2501/01C12N2501/11C12N2501/115C12N2506/45G01N33/5026A61P25/00C12N2506/03C12N2506/1346C12N5/0607
Inventor 丽贝卡·马顿塞尔久·P·帕斯卡
Owner THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
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