Method for identifying prenatal parental power relationship

A paternity relationship and location technology, applied in the field of effective prenatal paternity relationship identification, can solve the problems of increasing the difficulty of blood collection and increasing the difficulty of express delivery for customers, so as to improve practicability and practical operability, and reduce blood transportation. requirements, and the effect of low experimental environment requirements

Pending Publication Date: 2020-01-24
陈洪亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the storage temperature of plasma storage tubes is 6-37 degrees, and temperature control measures need to be taken in summer or winter in northern regions, which also increases the difficulty of e

Method used

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  • Method for identifying prenatal parental power relationship
  • Method for identifying prenatal parental power relationship
  • Method for identifying prenatal parental power relationship

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A technical scheme of a method for prenatal paternity identification, which includes the following steps:

[0036] Step 1, design sites and primers:

[0037] 1) Selection of sites: Select 13,000 sites in the human genome, and recommend the conditions for selecting sites:

[0038] 1. The minimum allele frequency MAF in the database is in the range of 0.2-0.5; 2. It conforms to Hardy-Weinberg equilibrium and the distance between any two markers is greater than 5Mb; 3. The loci are evenly covered on the whole genome. The above conditions are optional and only affect the accuracy and stability of the method;

[0039] 2) Design primers: according to the selected sites, design primers on the ION AMPLISEQ DESIGNER website. Primer or probe design can be selected from other suppliers.

[0040] Step 2, extract the sample DNA of the pregnant woman's sample and the undetermined male sample; wherein for the pregnant woman's sample, it can be a pregnant woman's peripheral blood or...

Embodiment 2

[0121] A technical scheme of a method for prenatal paternity identification, which includes the following steps:

[0122] Step 1, design sites and primers:

[0123] 1) Selection site: 13,000 sites are selected in the human genome, and the conditions for selecting the site are recommended: 1. The minimum allele frequency MAF in the database is in the range of 0.2-0.5; 2. It conforms to Hardy-Weinberg equilibrium and any two markers The distance between them is greater than 5Mb; 3. The sites evenly cover the whole genome. The above conditions are optional and only affect the accuracy and stability of the method;

[0124] 2) Design primers: according to the selected sites, design primers on the ION AMPLISEQ DESIGNER website. Primer or probe design can be selected from other suppliers.

[0125] Step 2, extract the sample DNA of the pregnant woman's sample and the undetermined male sample; wherein for the pregnant woman's sample, it can be a pregnant woman's peripheral blood or ...

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PUM

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Abstract

The invention relates to a method for identifying prenatal parental power relationship. Through cyclization amplification enlargement, only peripheral 10ul-1.2ml of plasma of a mother needs to be provided, and free DNA extracted from the peripheral plasma of the mother always contains free DNA of a foetus, so that only one part of samples of the mother and the foetus can be needed. Only the veinalblood of a pregnant woman needs to be extracted, the operation is simple and convenient, and the blood volume is small, so that the pregnant woman and the foetus cannot be damaged, and identificationcan be performed after pregnancy for 10 weeks.

Description

technical field [0001] The invention relates to the field of paternity identification, in particular to a method capable of using a small amount of blood plasma for effective prenatal paternity identification. Background technique [0002] The paternity test is based on the theory and practice of human genetics, analyzes the genetic characteristics from the similar characteristics of the morphological structure or physiological function of the offspring and the parent, judges the suspicious father-son relationship or mother-child relationship, and makes affirmative or negative conclusions . The earliest method for judging parentage to detect genetic polymorphisms is to use restriction endonucleases to perform restriction fragment length polymorphism analysis on variable number tandem repeats (VNTR) in the human genome. With the advancement of technology, DNA polymerase chain reaction (PCR), so that shorter nucleic acid fragments can also be used for analysis, the analysis t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6858G16B30/10G16B40/00
CPCC12Q1/6858G16B30/10G16B40/00C12Q2525/191C12Q2525/307C12Q2531/113
Inventor 陈洪亮
Owner 陈洪亮
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