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Process for optimizing sulfation of Scrophularia ningpoensis polysaccharide by utilizing response surface method

A technology of polysaccharide sulfate and response surface methodology, applied in the biological field, can solve the problems of few reports on the antioxidant activity of Scrophulariae polysaccharides in vitro, few studies on the pharmacological activity of Scrophulariaceae polysaccharides, etc., and achieve the effect of improving the clearance rate of ABTS+

Inactive Publication Date: 2020-02-11
JINING MEDICAL UNIV
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Problems solved by technology

[0008] At present, the research focus of Scrophulariae Scrophulariae is mostly on the iridoid glycosides and their activities, but there are few reports on the extraction and separation of polysaccharides from Scrophulariae Scrophulariae and its in vitro antioxidant activity, and more studies on the pharmacological activities of Scrophulariae polysaccharides in vivo. as rare

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  • Process for optimizing sulfation of Scrophularia ningpoensis polysaccharide by utilizing response surface method
  • Process for optimizing sulfation of Scrophularia ningpoensis polysaccharide by utilizing response surface method
  • Process for optimizing sulfation of Scrophularia ningpoensis polysaccharide by utilizing response surface method

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Embodiment Construction

[0128] A technique for optimizing scrophulariaceae polysaccharide sulfation using response surface methodology, comprising the following steps:

[0129] ⑴Sample preparation:

[0130] Dry the dried roots of Scrophulariaceae at 50°C for 24 hours and crush them to 10 meshes. Add deionized water to soak for 1 hour according to the ratio of 200g: 6L of material to liquid, decoct for 2 hours, filter the decoction, and decoct the dregs again. Boil once and combine the filtrates; after the filtrates are concentrated to 350~450 mL by a rotary evaporator under reduced pressure, a concentrated solution is obtained, and anhydrous ethanol 4 times its volume is added to the concentrated solution, mixed well, left standing overnight, and discarded. The precipitate was cleared to obtain a precipitate; 100 mL of deionized water was added to the precipitate to dissolve, and the pigment was removed by passing through a macroporous resin column DM-130. The eluate was concentrated by a rotary evap...

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Abstract

The invention relates to a process for optimizing sulfation of Scrophularia ningpoensis polysaccharide by utilizing a response surface method. The process comprises the following steps: (1) preparation of a sample: preparing Scrophularia ningpoensis polysaccharide powder from dry roots of Scrophularia ningpoensis, and then carrying out reaction extraction according to different reaction temperatures, reaction time and esterification reagent proportions to obtain Scrophularia ningpoensis polysaccharide sulfate powder corresponding to different proportions of esterification reagents; (2) determination of the substitution degree of a sample through barium chloride-gelatin turbidimetry; (3) determination of the ABTS<+> removal rate of the sample; (4) experimental design and statistical analysis: 1, single-factor test, and 2, response-surface-method optimization design: establishing a multivariate quadratic regression equation by taking reaction temperature, reaction time and the esterification reagent proportions as investigation factors according to the results of the single-factor test; and (5) analysis and optimization of an experimental result: carrying out drawing analysis by utilizing Design Expert 8.0.6 software according to the multivariate quadratic regression equation to obtain a response surface of the regression equation and a contour map of the response surface. The method is simple to operate and high in extraction rate.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a process for optimizing the sulfation of crocus polysaccharides by using the response surface method. Background technique [0002] Scrophulariaceae ( Scrophulariae Radix ) for Scrophulariaceae ( Scrophulariaceae ) Plant Scrophulariaceae ( Scrophularia ningpoensis Hemsl . ) of the dried roots. It is mainly produced in Zhejiang, Hubei, Jiangsu, Jiangxi and other provinces, among which the Scrophulariaceae produced in Zhejiang is the best product, and it is recognized as one of the eight flavors of Zhejiang. It tastes sweet, bitter, salty, and slightly cold. It has the effects of clearing heat and cooling blood, purging fire and detoxifying, nourishing yin and reducing fire, etc. Injury constipation embolism. At present, it is believed that its main chemical components include iridoids, phenylpropanoid glycosides, phenylethanol glycosides, sterols, flavonoids and polysaccharid...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00G16C20/10
CPCC08B37/0003C08B37/006G16C20/10
Inventor 王建安高丽娜任强王慧云李昱周利润
Owner JINING MEDICAL UNIV
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