A kind of electrochemical immunosensor and its preparation method and application

An immunosensor, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of cumbersome process, low sensitivity, and the modification method needs to be developed, and achieves improved selectivity, good stability and reproducibility. sexual effect

Active Publication Date: 2022-03-22
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional electrode modification method needs to modify the electrode surface layer by layer, the process is cumbersome, and the sensitivity is low. New modification methods need to be developed

Method used

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  • A kind of electrochemical immunosensor and its preparation method and application
  • A kind of electrochemical immunosensor and its preparation method and application
  • A kind of electrochemical immunosensor and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method of an electrochemical immunosensor, comprising the following steps:

[0043] (1) Add 1.00 g of cetyltrimethylammonium bromide (CTAB) and 3.5 mL of 2.00 mol / L sodium hydroxide solution to 480 mL of ultrapure water, and stir vigorously at 80 ° C for 30 minutes;

[0044] (2) Slowly add 5.00 mL of tetraethyl orthosilicate (TEOS) dropwise while vigorously stirring, and mix and react for 2 hours to obtain product A, which is washed with ethanol for later use;

[0045] (3) product A after washing in step (2) is dispersed in the mixed solution of 70mL methanol and 3.0mL 37.2% hydrochloric acid, reflux twice, each ten minutes, centrifugation obtains product MSN and washes with methanol, will wash after The product MSN was dried under vacuum at 60°C for later use;

[0046] (4) Ultrasonic disperse the product MSN after vacuum drying in step (3) in a mixture of 1.0mL 3-aminopropyltriethoxysilane (APTES) and 50.0mL methanol, reflux at 80°C for 8 hours, and cent...

Embodiment 2

[0057] The surface of the glassy carbon electrode modified in Example 1 was characterized by a scanning electron microscope. Such as figure 2 As shown, it can be seen from the figure that carboxylated graphene is attached to the surface of the glassy carbon electrode, and carboxylated graphene can be connected to Janus through amino groups. The sensitivity has been greatly improved. It shows that the aptamer / amino Janus particle immune electrode prepared by the present invention is successfully modified.

Embodiment 3

[0059] Characterization of the modification process of the immunoelectrochemical sensor prepared in Example 1:

[0060] Get the Janus particle modified electrode that embodiment 1 prepares, in the phosphate buffer of 0.1mM pH 7, with 10mM [Fe(CN) 6 ] 3- / 4- As a probe, the modification process of the electrode was characterized by cyclic voltammetry. Such as image 3 Shown: The peak current generated by the electrode decreases after the Janus particles are modified. This is because the Janus particles are connected to the carboxylated graphene and attached to the surface of the electrode, and polystyrene is exposed in the potassium ferricyanide solution, so its conductivity will decrease. The peak current detected after soaking in the ractopamine aptamer decreased, indicating that the ractopamine antibody was well attached to the surface of the gold nanoparticles. After soaking in the bovine serum albumin solution, the current decreased to a certain extent, which proved tha...

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Abstract

The invention provides an electrochemical immunosensor and its preparation method and application. An electrochemical immunosensor is prepared by using aptamer / amino group as a functional group to prepare Janus particles, and using it to modify a glassy carbon electrode to prepare electrochemical immunosensor. The electrochemical immunosensor prepared by the method can be used for the detection of β-receptor agonist ractopamine. The method improves the sensitivity and selectivity of the electrochemical immunosensor electrode, makes the electrode modification process easier, has good stability and reproducibility, and can be applied to the detection of ractopamine in human urine.

Description

technical field [0001] The invention relates to the technical field of preparation of electrochemical electrode materials, in particular to a preparation method of an electrochemical immunosensor and the application of the prepared sensor in detecting ractopamine in human urine. Background technique [0002] Electrochemical immunosensor combines the high specificity of immune reaction with the high sensitivity of electrochemical analysis. Because of its advantages of small sample consumption, high sensitivity and low cost of instruments, it has become a new method in clinical, biomedicine, environmental chemistry and food safety analysis. a powerful analytical tool. Among them, electrode modification is the key to the preparation of immunoelectrochemical sensors. The traditional electrode modification method requires layer-by-layer modification of the electrode surface, which is cumbersome and has low sensitivity. New modification methods need to be developed. [0003] Jan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48G01N33/543G01N33/531
CPCG01N27/308G01N27/3277G01N27/3278G01N27/48G01N33/5438G01N33/54346G01N33/54393G01N33/531
Inventor 周影双少敏
Owner SHANXI UNIV
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