38results about How to "The grooming process is simple" patented technology

The invention provides a modification method of a ceramic membrane and a modified ceramic membrane. The modification method of the ceramic membrane comprises the following steps: performing surface treatment on the ceramic membrane to obtain a fresh ceramic membrane; coating the surface of the ceramic membrane with a silane compound through an impregnation method or a sedimentation method; performing thermal treatment on the coated ceramic membrane under a protective atmosphere, naturally cooling to obtain the modified ceramic membrane after the modification. The modification method of the ceramic membrane provided by the invention has advantages that the modification process is simple, the raw material cost is low, and secondary pollution on the environment can be avoided in the modification process; compared with the common organic modification material, the modified ceramic membrane prepared through modification has the features of high long-term stability, strong acid-base resistance, and strong hydrophobic property; and the modified ceramic membrane can be extensively applied to sea water desalinization, oil-water separation, wastewater treatment and other fields.

The invention relates to a non-covalently modified graphene field effect transistor-based tumor marker detection sensor and a production method thereof. The sensor comprises a silica substrate, a graphene channel and a reaction chamber. The production method comprises the following steps: making a metal electrode, carrying out wet transfer on graphene, making the graphene channel and a protection insulation layer of the graphene channel, and modifying the surface of graphene in a non-covalent mode. The sensor has the advantages of convenient making process, simple modification process, easy operation, and high sensitivity and good specificity in application; and the making method can also be used to make most of biosensors of capture probes with amino groups, and the sensor is used to detect target molecules.

The invention provides a preparation method of an electrochemical aptamer sensor for detecting aflatoxin B1. The preparation method comprises the following steps of (1) dropwise adding mesoporous carbon onto a work electrode; (2) performing potentiostatic gold nanoparticle deposition for modifying CMK / GCE; (3) dropwise adding an AFB1 aptamer modified with sulfydryl at one end onto an AuNPs / CMK / GCEelectrode; (4) immersing the Apt / AuNPs / CMK / GCE into an MB (Methylene Blue) solution, so that the MB is adsorbed on an aptamer chain to obtain an MB-Apt / AuNPs / CMK / GCE electrode; and (5) detecting thereduction peak current value. The electrochemical aptamer sensor for detecting aflatoxin B1 can achieve the goal of quantitatively detecting a target object; the characteristics of high speed and operation simplicity are realized; and the defects of operation complexity and long detection period of a large-scale detecting instrument are overcome.

The invention relates to a method of fast enrichment and fast identification on the phosphoeptide of the modificatory sample target by the matrix-assisted laser-desorption / time-of-flight mass spectrometry (MALDI-TOF-MS), which is to physically adsorb the fine magnetic nano particle onto the sample target, wash the sample after the sample is loaded to remove the non phosphoeptide and phosphate, add the matrix to make cocrystallization with the phosphoeptide absorbed on the sample target and then conduct the MALDI-TOF-MS analysis. The method does not relate to the chemical reaction without pre-processing to the target, which can modify the target MALDI made of different substrate materials; A plurality of samples complete their enrichment at the same time on the modified target MALDI; There are no sample transfer in the whole process from the sample loading to the mass spectrometric analysis. The invention is easy, fast and sensitive to operate, which has a good application value in the large-scale analysis of the phosphoproteome.

The invention discloses a portable sensing system for early diagnosing liver cancer and functional modification method of the portable sensing system. The system comprises a micro cantilever array sensing unit, a surface modifying layer, an encapsulation shell, a driving module and a digital display interface processing circuit, wherein the micro cantilever array sensing unit is used for converting a mechanical signal of a cantilever into an electric signal and outputting the electric signal to a digital display interface processing circuit; the surface modifying layer grows on the surface of the micro cantilever array sensing unit and is used for functionalizing the surface of the micro cantilever array sensing unit so that the micro cantilever array sensing unit is capable of combining with a liver cancer landmark antigen specificity; the encapsulation shell is used for protecting the micro cantilever array sensing unit and providing a biochemical reaction sample inlet / outlet and a reaction chamber; the driving module is used for providing a resonant drive signal; and the digital display interface processing circuit is used for obtaining and outputting the liver cancer landmark quality according to the variation of resonant frequency of the micro cantilever. According to the system and method provided by the invention, the early screening of the liver cancer is possible, and the important significance is provided for early diagnosing and early treating the liver cancer and improving the survival rate of the liver rate.

The invention belongs to a technical field of preparation of electrochemical electrode materials, and provides an electrochemical sensor for simultaneously detecting guanine and adenine. The electrochemical sensor based on copper@nickel / nitrogen, boron doped reduction graphene oxide simultaneously detects guanine and adenine. The electrochemical sensor is based on copper@nickel / nitrogen, boron doped reduction graphene oxide composites, and prepared by modifying a surface of glassy carbon electrode. The electrochemical sensor has high sensitivity and selectivity, good stability and reproducibility, and the electrode modification process is simpler. The preparation method comprises: based on the copper@nickel / nitrogen, boron doped reduction graphene oxide composites, preparing the nitrogen,boron doped reduction graphene oxide N, B-rGO by hydrothermal method, and preparing the copper@nickel / nitrogen, boron doped reduced graphene oxide Cu@Ni / N, B-rGO; and preparing an electrode. The invention improves the sensitivity of the electrode and makes the electrode modification process simpler. The electrochemical sensor is used to construct a sensing system for simultaneous detection of guanine and adenine in adenosine, which significantly increases electrode selectivity.

The invention provides an electrochemical immunosensor as well as a preparation method and an application thereof. Janus particles with hydrophilic / hydrophobic properties are prepared with aptamer / octadecanethiol as functional groups and are used for modifying a glassy carbon electrode, and the electrochemical immunosensor is prepared. The sensor prepared with the method can be used for detecting beta-receptor agonist ractopamine. By means of the method, the sensitivity and the selectivity of the electrode are improved, so that the electrode modification process is simpler and more convenient, and the electrochemical immunosensor has good stability and reproducibility and can be applied to detection of ractopamine in human urine.

The invention discloses an electrochemical sensor for quick detection of plasma indole. The electrochemical sensor for quick detection of plasma indole is constructed by modification of a multiwall carbon nanotube-chitosan compound on the surface of a screen-printed electrode. By combination of advantages of simplicity and convenience in operation and quick in response in electrochemical detection and electron transfer acceleration effect of nanometer materials, the electrochemical sensor realizes quantitative determination of plasma indole concentration according to a linear relation between electrochemical signals and the concentration of to-be-detected materials. The electrochemical sensor has advantages of high sensitivity, quickness in response, convenience in preparation, low cost, disposability and availability for batch production.

The invention discloses a solution organic light emitting diode based on a silver nanocube, comprising a sequentially laminated anode, a hole transport layer, a light emitting layer containing silvernanocube, an electron transport layer, and a cathode. The invention also discloses a preparation method of a solution organic light emitting diode based on silver nanocube: firstly, the ITO glass substrate is thoroughly cleaned, then a hole transport layer is spin-coated to prepare the hole transport layer, the hole transport layer comprises a silver nanocube light emitting layer, and then an electron transport layer and a metal electrode are sequentially prepared by evaporation in vacuum. The invention has the advantages that the silver nano cube is dispersed in the light emitting layer, theluminous efficiency of the organic light emitting diode is effectively improved by utilizing the plasma resonance effect of the silver nano cube, the preparation method is simple and easy, and has potential application value.

The invention discloses a modifier, which comprises a structural unit represented by a general formula (I), wherein R1 represents a direct bond or C1-C5 alkylidene or substituted alkylidene, R2 represents a direct bond or C1-C5 alkyl or substituted alkyl, R3 is a direct bond or C1-C5 alkyl or substituted alkyl, R4 is a direct bond, C1-C5 alkylidene or substituted alkylidene or C1-C5 acyloxy, R5 isa zwitterionic group, and the molecular weight of the modifier is 10-180 KDa. The invention also discloses a preparation method and a use method of the modifier, and a medical material.

A clone for screening tumor necrosis target antibody, its preparing method and use are disclosed. It includes: selecting autoimmune disease patient, extracting B lymph cell and phlogocyte with high-expressing auto-antibody, extracting RNA, amplifying and cloning antibody gene, constructing superficial displaying library by Helper protobiology, multiple screening this library, removing gpIII gene fragment, converting competent bacteria dissolving expressing after re-connecting, selecting single-cloning antibody and enriching expressing, selecting positive clone for screening tumor necrosis target antibody. It achieves low cost, simple operation and rapid industrial method.

The invention relates to an adapter-based optical chemical biosensor and a method thereof for detecting a target molecule, in particular to an optical chemical biosensor used for identifying a targetmolecule for example, a polydiacetylene structure organized molecular assembly of nucleotide, amino acid, polypeptide, protein, entire cell, other specific molecules and the like, by using an adapter,and a method for detecting the target molecule by using the optical chemical biosensor for the polydiacetylene structure organized molecular assembly. In the optical chemical biosensor, an adapter for a specific binding target molecule is combined on the surface of the polydiacetylene structure organized molecular assembly so that the specific target molecule can be detected / identified through the changed ultraviolet-visible light absorbency or fluorescence intensity of the polydiacetylene structure organized molecular assembly when the specific target molecule is exposed outside the corresponding adapter.

The invention provides an electrochemical immunosensor as well as a preparation method and application thereof. According to the electrochemical immunosensor, Janus particles are prepared by using anaptamer / amino as a functional group and are used for modifying a glassy carbon electrode, thereby preparing the electrochemical immunosensor. The prepared electrochemical immunosensor can be used fordetecting beta-receptor stimulant ractopamine. With the method, the sensitivity and selectivity of the electrochemical immunosensor electrode are improved; the electrode modification process is simpler and more convenient; and the method has high stability and reproducibility and can be applied to detection of ractopamine in human urine.

The invention relates to an indole electrochemical sensor constructed based on a nitrogen-doped ordered mesoporous carbon modified electrode and application of the indole electrochemical sensor, and belongs to the field of electrochemical detection. A compound formed by dispersing nitrogen-doped ordered mesoporous carbon in chitosan is modified on the surface of a screen-printed electrode, and because of high specific surface area and good conductivity of a nitrogen-doped ordered mesoporous carbon material as well as the characteristics of quick response, simplicity and convenience in operation and the like of the electrochemical detection method, an electrochemical sensor for detecting the plasma indole is constructed. Electrochemical detection is carried out by adopting differential pulse voltammetry, and a standard curve is drawn according to a linear relationship between an electrochemical response signal and the concentration of a to-be-detected substance, so that quantitative analysis is carried out on plasma indole. The indole electrochemical sensor for detecting the plasma indole has the advantages of simplicity and convenience in preparation, low cost, high sensitivity, strong anti-interference capability and the like.

The invention belongs to the technical field of preparation of electrochemical electrode materials, and provides an electrochemical sensor for detecting glucose and a preparation method thereof. The electrochemical sensor is an electrochemical sensor based on cobalt@nickel-metal organic framework-graphene (CNMs-GR) , the sensor was used to simultaneously detect glucose in human serum. It exhibits high sensitivity and selectivity, the electrode modification process is simpler, and it has good stability and reproducibility. Based on cobalt@nickel‑metal organic framework‑graphene (CNMs‑GR) nanocomposites, bimetallic frameworks were synthesized by a simple hydrothermal reaction to prepare cobalt@nickel‑metal organic framework‑graphene CNMs‑GR nanocomposites. The sensitivity of the electrode is improved and the electrode modification process is simplified. The electrochemical sensor is used to construct a sensing system for detecting glucose in human serum, which significantly improves the selectivity of the electrode. The detection limit is 0.087μmol / L. Wide linear range and high sensitivity.

The invention provides an electrochemical immunosensor as well as a preparation method and an application thereof. Janus particles with hydrophilic / hydrophobic properties are prepared with aptamer / octadecanethiol as functional groups and are used for modifying a glassy carbon electrode, and the electrochemical immunosensor is prepared. The sensor prepared with the method can be used for detecting beta-receptor agonist ractopamine. By means of the method, the sensitivity and the selectivity of the electrode are improved, so that the electrode modification process is simpler and more convenient, and the electrochemical immunosensor has good stability and reproducibility and can be applied to detection of ractopamine in human urine.

The present invention provides a preparation method for detecting aflatoxin B1 electrochemical aptamer sensor, comprising the following steps, 1) adding mesoporous carbon to the working electrode; 2) depositing gold nanoparticles at a constant potential to CMK / GCE Modification; 3) Drop AFB1 aptamer modified with a sulfhydryl group on the AuNPs / CMK / GCE electrode; 4) Immerse Apt / AuNPs / CMK / GCE in methylene blue (MB) solution, and adsorb MB to the aptamer chain Get the MB‑Apt / AuNPs / CMK / GCE electrode; 5) Detect the reduction peak current value. The aflatoxin B1 electrochemical aptamer sensor created by the invention can achieve the purpose of quantitative detection of the target object, and the characteristics of fastness and simple operation overcome the disadvantages of complex operation and long detection cycle of large-scale detection instruments.

The invention relates to an electrochemical sensor, device and method for detecting cyclovirobuxine D. The electrochemical sensor comprises a glassy carbon electrode, a single-walled carbon nanotube is modified on the surface of the glassy carbon electrode, and polybromothymol blue is modified on the single-walled carbon nanotube. According to the invention, polybromothymol blue is used as a recognition element and an electrochemical probe of cyclovirobuxine D at the same time to construct the electrochemical sensor of cyclovirobuxine D, and quantitative analysis can be carried out on cyclovirobuxine D through the current change of the electrochemical sensor before and after the action with cyclovirobuxine D. The electrochemical sensor and the device are simple in preparation process, lowin cost, convenient to operate, high in analysis speed, sensitive in response, easy to update and reusable, and quantitative analysis of cyclovirobuxine D in various physiological samples can be realized. The electrochemical sensor, device and method provided by the invention have a wide application prospect in quality standard research of the cyclovirobuxine D and physiological and pathological events related to the cyclovirobuxine D.

The invention discloses a modifier, which comprises a structural unit represented by a general formula (I), wherein R1 represents a direct bond or C1-C5 alkylidene or substituted alkylidene, R2 represents a direct bond or C1-C5 alkyl or substituted alkyl, R3 is a direct bond or C1-C5 alkyl or substituted alkyl, R4 is a direct bond, C1-C5 alkylidene or substituted alkylidene or C1-C5 acyloxy, R5 isa zwitterionic group, and the molecular weight of the modifier is 10-180 KDa. The invention also discloses a preparation method and a use method of the modifier, and a medical material.

An electrochemical immunosensor based on Janus particles and its preparation method and application, belonging to the field of electrochemical electrode material preparation technology and food toxin detection, the purpose is to provide an electrochemical immunosensor, which is functionalized with aptamers / amino groups The Janus particles were prepared by the group and used to modify the surface of glassy carbon electrodes for the detection of ochratoxin A in red wine. The prepared electrochemical immunosensor has good stability and reproducibility, and can detect ochratoxin A in actual red wine.

The invention relates to a graphene tumor marker sensor based on a multifunctional nanoscale protein film and a preparation method thereof. The sensor comprises graphene, the multifunctional nanoscale protein film and a reaction chamber transferred onto a silicon dioxide substrate. The preparation method comprises the steps as follows: 1, the graphene is transferred to the surface of a titanium electrode; 2, the inactivated BSA protein film modifies the graphene; 3, photoetching and etching are performed; 4, a protective insulating layer is prepared; and 5, antibodies are coupled. The graphene tumor marker sensor provided by the invention is simple and easy to operate during a modification process, strong in universality, can perform different modifications according to different needs to achieve detection with high sensitivity and specificity on different target molecules, and has good application prospects.

The invention discloses a use of a copper-adenine coordination polymer in electrochemical sensing. The copper-adenine coordination polymer has a chemical formula of Cu2(N3, N9-Ade)4(H2O)2. 5H2O. An electrode is prepared from the copper-adenine coordination polymer and through a three-electrode system, phenformin hydrochloride is detected through a differential pulse voltammetry method. Through a cyclic voltammetry method and a chronoamperometry method, hydrogen peroxide is detected. The electrode modification process is simple and is free of a toxic substance CdS. The modified electrode can be used for detecting hydrogen peroxide and phenformin hydrochloride and realizes double functions of a sensor.