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Graphene tumor marker sensor based on multifunctional nanoscale protein film and preparation method thereof

A tumor marker, nano-scale technology, applied in the direction of instruments, scientific instruments, biological testing, etc., can solve the problems of difficult control of adsorption amount, easy introduction of false positive signals, etc., and achieve good application prospects, strong versatility, and improved performance Effect

Active Publication Date: 2017-09-15
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

These non-covalent cross-linking agents are usually adsorbed on the surface of graphene through self-assembly, but there

Method used

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  • Graphene tumor marker sensor based on multifunctional nanoscale protein film and preparation method thereof
  • Graphene tumor marker sensor based on multifunctional nanoscale protein film and preparation method thereof
  • Graphene tumor marker sensor based on multifunctional nanoscale protein film and preparation method thereof

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Embodiment 1

[0031] Such as figure 1 Shown, the preparation process of this embodiment is as follows:

[0032] With 300nm silicon dioxide on the silicon substrate, titanium gold electrodes are made on the surface of silicon dioxide by lift-off process as the drain and source respectively, and single-layer graphene is transferred on the surface of the gold electrode. The process steps Such as figure 2 (a) shown. Add the BSA solution dropwise on the graphene surface, and inactivate the graphene surface at 80°C for 3 minutes to form an inactivated BSA protein film. The process steps are as follows: figure 2 (b) shown. The size of the graphene channel is determined by photolithography and the part other than the channel is etched away by oxygen plasma etching. The process steps are as follows figure 2 (c) and figure 2 (d) shown. Finally, acetone is used to dissolve the photoresist for channel protection. The process is as follows: figure 2 As shown in (e), the SU-8 photoresist is ...

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Abstract

The invention relates to a graphene tumor marker sensor based on a multifunctional nanoscale protein film and a preparation method thereof. The sensor comprises graphene, the multifunctional nanoscale protein film and a reaction chamber transferred onto a silicon dioxide substrate. The preparation method comprises the steps as follows: 1, the graphene is transferred to the surface of a titanium electrode; 2, the inactivated BSA protein film modifies the graphene; 3, photoetching and etching are performed; 4, a protective insulating layer is prepared; and 5, antibodies are coupled. The graphene tumor marker sensor provided by the invention is simple and easy to operate during a modification process, strong in universality, can perform different modifications according to different needs to achieve detection with high sensitivity and specificity on different target molecules, and has good application prospects.

Description

technical field [0001] The invention belongs to the field of graphene biosensors, in particular to a graphene tumor marker sensor based on a multifunctional nanoscale protein film and a preparation method thereof. Background technique [0002] With the emergence of nanomaterials such as nanowires, carbon nanotubes, and graphene, the detection of biomolecules can be more effectively realized by using the charge of the organism itself through electrical detection methods. The choice of sensing materials in the biosensing interface plays a critical role in the construction of highly sensitive and affordable biosensors. Two-dimensional graphene material has sp 2 The hybridized carbon atoms are arranged in a single-layer atomic structure in a honeycomb lattice. Excellent mechanical, optical, electrical properties and high specific surface area make graphene an ideal bioelectronic sensing material. Graphene-based biosensors have been widely used in the detection of biomolecules...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N27/327
CPCG01N27/3275G01N33/57473G01N33/6893
Inventor 毛红菊周麟王琨金庆辉赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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