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Electrochemical sensor for quick detection of plasma indole

An electrochemical and sensor technology, applied in the field of electrochemical detection, achieves the effects of simple operation, simple operation, simple production and modification process

Active Publication Date: 2017-06-27
重庆医科大学国际体外诊断研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant research reports on the preparation method and application of electrochemical sensors for the detection of plasma indole by electrochemical analysis methods at home and abroad.

Method used

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  • Electrochemical sensor for quick detection of plasma indole
  • Electrochemical sensor for quick detection of plasma indole
  • Electrochemical sensor for quick detection of plasma indole

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the preparation method of the electrochemical sensor that detects plasma indole

[0038] 1) Prepare the screen printing electrode of the present invention:

[0039] The screen printing electrode of the present invention prints carbon paste, silver / silver chloride paste and insulating paste sequentially on a polyethylene terephthalate (PET) substrate. Specifically include the following steps:

[0040] ①Clean the PET substrate, print carbon paste on the PET substrate after drying, make working electrodes and auxiliary electrodes, and dry at room temperature;

[0041] ②Print silver paste containing silver chloride on the above-mentioned PET substrate to make a reference electrode and dry at room temperature;

[0042] ③Avoid the circular working area, print insulating paste on the above PET substrate to cover the wires;

[0043] ④The above-mentioned working electrode, auxiliary electrode and reference electrode form a circular working area, and each electro...

Embodiment 2

[0051] Embodiment 2: the use method of the electrochemical sensor that detects plasma indole

[0052]1) Purchasing analytically pure ether for pretreatment of plasma samples;

[0053] 2) Prepare phosphate buffer: prepare 0.1mol L respectively -1 Sodium dihydrogen phosphate and disodium hydrogen phosphate solution are mixed according to a certain volume ratio to prepare a pH=7.0 phosphate buffer solution, which is placed in a container;

[0054] 3) Take 300 μL of plasma sample and 600 μL of the ether solution in a test tube, shake on a shaker at 250 rpm for 18 minutes, then centrifuge at 13300 g at 4°C for 5 minutes, and finally take the supernatant and dry it with nitrogen at 30°C;

[0055] 4) Reconstitute the treated sample with 150 μL ultrapure water;

[0056] 5) Take equal volumes of the solution and phosphate buffer solution obtained in step 3) respectively, vortex and mix to obtain a reaction mixture;

[0057] 6) Connect the screen printing electrode to CHI852C electro...

Embodiment 3

[0059] This example is to investigate the electrochemical response to indole before and after the screen printing electrode modification. Use the bare electrode and the modified electrode to measure the indole standard solution respectively, the results are shown in figure 2 . The dotted line a and the short horizontal line b in the figure represent the measurement of phosphate buffer and 100.0 μg L -1 The voltammetric curve of the indole standard, the dotted line c and the solid line d in the figure respectively represent the determination of phosphate buffer and 100.0 μg L by the modified electrode -1 Voltammetric curves of indole standards. The results showed that the obtained electrochemical signal was produced by the oxidation of indole. In addition, the electrochemical response signal of indole measured by the modified electrode was significantly improved, and the peak shape of the voltammetry curve was obviously improved.

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Abstract

The invention discloses an electrochemical sensor for quick detection of plasma indole. The electrochemical sensor for quick detection of plasma indole is constructed by modification of a multiwall carbon nanotube-chitosan compound on the surface of a screen-printed electrode. By combination of advantages of simplicity and convenience in operation and quick in response in electrochemical detection and electron transfer acceleration effect of nanometer materials, the electrochemical sensor realizes quantitative determination of plasma indole concentration according to a linear relation between electrochemical signals and the concentration of to-be-detected materials. The electrochemical sensor has advantages of high sensitivity, quickness in response, convenience in preparation, low cost, disposability and availability for batch production.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, in particular to an electrochemical sensor for detecting plasma indole. Background technique [0002] Indole is a product of tryptophan metabolized by intestinal flora and widely exists in the intestinal tract. It is currently known that indole is a signaling molecule, which has two main functions in the body. On the one hand, it is involved in maintaining the homeostasis of intestinal flora, controlling the functions of various bacteria, such as promoting biofilm formation, maintaining the stability of plasmids, regulating bacterial division and growth, and inducing antibiotic resistance; on the other hand, Participate in the regulation of human physiological functions, specifically in the following points: (1) avoid bacterial infection in the body; (2) regulate the expression of inflammatory / anti-inflammatory genes and enhance intestinal barrier function; (3) participate in the regulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26
CPCG01N27/26G01N27/30
Inventor 张晓清金明超丁敏史争虎何一帆邹文毕
Owner 重庆医科大学国际体外诊断研究院
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