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Electrochemical sensor, device and method for detecting cyclovirobuxine D

A cycloviral buxicine and detection method technology, which is applied in the field of analytical chemistry, can solve the problems of high analysis cost, large amount of reagent consumption, and complicated preparation process, and achieve simple modification and preparation process, small amount of reagent and sample consumption, and simple equipment Effect

Active Publication Date: 2021-01-05
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

These methods all have some limitations: due to the secondary amino group contained in the Cyclovirubuxin D molecule, which is basic, if the silanol group on the surface of the stationary phase of the reversed-phase chromatographic column is not completely bonded and the coverage of the stationary phase is small, The exposed silanol groups can react with basic groups to cause adsorption or ion exchange, causing peak tailing and reducing peak heights, thereby affecting accurate quantification and reducing sensitivity; The retention is weak on the commonly used reversed-phase C18 column, and ion-pair reagents are usually added to the mobile phase to enhance its retention, but the column requires a long equilibration time and the analysis speed is slow
However, these derivatization reagents are highly toxic, long-term use will cause great harm to the human body, and the generated derivative by-products will inevitably interfere with chromatographic analysis.
In ELSD detection, non-volatile salts cannot be used as the mobile phase, which is incompatible with most ion-pair reagents, which limits the choice of mobile phase; the baseline noise is large, the sensitivity is low, and the response is not linear, but logarithmic and linear The range is narrow, in addition, a gas source with stable flow and high purity is required, and the analysis cost is high
However, the mass spectrometer has a large volume, high price and maintenance costs, high requirements for operators, and poor popularity
The mobile phase of liquid chromatography-mass spectrometry cannot use non-volatile buffer salts; liquid chromatography-mass spectrometers commonly use electrospray ionization (ESI) ion sources, which will produce matrix effects when analyzing complex systems (such as physiological samples), resulting in quantitative Bias in the results of the analysis
The currently reported high-performance liquid chromatography-electrochemical detection uses a boron-doped diamond (BDD) electrode as the working electrode, and the preparation of the BDD film has harsh experimental conditions and a complicated preparation process.
Therefore, the above-mentioned analysis method based on separation and subsequent detection has expensive instruments and equipment, high analysis cost, large amount of reagents, unfriendly environment, complex operation and time-consuming operation, and cannot realize the simple and fast detection of Cyclovitamin D

Method used

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  • Electrochemical sensor, device and method for detecting cyclovirobuxine D
  • Electrochemical sensor, device and method for detecting cyclovirobuxine D
  • Electrochemical sensor, device and method for detecting cyclovirobuxine D

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Experimental program
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Effect test

Embodiment 1

[0050] Carbon nanotube modified glassy carbon electrode and poly-bromothymol blue / carbon nanotube modified glassy carbon electrode are used for electrochemical measurement of cycloviric buxillus D according to the following scheme:

[0051] (1) preparing glassy carbon electrodes modified by carbon nanotubes;

[0052] (2) Carrying out the electrochemical measurement of ring-dimensional buxillus D with the glassy carbon electrode modified by carbon nanotubes in step (1) as the working electrode;

[0053] (3) prepare poly-bromothymol blue / carbon nanotube modified glassy carbon electrode;

[0054] (4) Using the glassy carbon electrode modified by poly-bromothymol blue / carbon nanotubes in step (3) as the working electrode, the electrochemical measurement of Cyclovitamin D was carried out.

[0055] Wherein, the glassy carbon electrodes modified by carbon nanotubes in the step (1) are prepared in 3 groups according to the following method:

[0056] Group 1: Polish the glassy carbon...

Embodiment 2

[0069] A glassy carbon electrode modified with poly-bromothymol blue / carbon nanotubes was used as the working electrode, Ag / AgCl (1M KCl) was used as the reference electrode, and a platinum counter electrode was used as the counter electrode.

[0070] (1) The glassy carbon electrode modified with poly-bromothymol blue / carbon nanotubes was firstly subjected to differential pulse scanning in 0.1M phosphate buffer (pH=7.4), and then placed in 0.1 Differential pulse scans were performed in M ​​phosphate buffer (pH=7.4). After taking it out, the electrode is at 50U·mL -1 After soaking in heparin solution for 5 minutes, differential pulse scanning was performed in blank 0.1M phosphate buffered saline.

[0071] (2) Repeat step (1) seven times, the experimental results are as follows figure 2 As shown in A.

[0072] (3) The poly-bromothymol blue / carbon nanotube-modified glassy carbon electrode was firstly subjected to differential pulse scanning in 0.1M phosphate buffer (pH=7.4) ...

Embodiment 3

[0076] (1) Repeat step (3) of Example 1.

[0077] (2) The poly-bromothymol blue / carbon nanotube-modified glassy carbon electrode obtained in step (1) was first immersed in a 0.1M blank phosphate buffer solution (pH=7.4) for differential pulse scanning. Subsequently, the poly-bromothymol blue / carbon nanotube-modified glassy carbon electrode was immersed in a 0.1M phosphate buffer solution of cyclovirbuxine D at different concentrations (0.01-50 μM) for 20 minutes, and then the electrode was taken out Wash with secondary water to remove the cycloviral buxillus D physically adsorbed on the working electrode, and then place it in the blank phosphate buffer solution for differential pulse scanning. The results are as follows: image 3 As shown in A.

[0078] (3) the relative value I of the peak current change on the differential pulse voltammetry curve recorded by the electrode in step (2) 0 -I / I 0 The linear correction was performed on the poly-bromothymol blue / carbon nanotube ...

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Abstract

The invention relates to an electrochemical sensor, device and method for detecting cyclovirobuxine D. The electrochemical sensor comprises a glassy carbon electrode, a single-walled carbon nanotube is modified on the surface of the glassy carbon electrode, and polybromothymol blue is modified on the single-walled carbon nanotube. According to the invention, polybromothymol blue is used as a recognition element and an electrochemical probe of cyclovirobuxine D at the same time to construct the electrochemical sensor of cyclovirobuxine D, and quantitative analysis can be carried out on cyclovirobuxine D through the current change of the electrochemical sensor before and after the action with cyclovirobuxine D. The electrochemical sensor and the device are simple in preparation process, lowin cost, convenient to operate, high in analysis speed, sensitive in response, easy to update and reusable, and quantitative analysis of cyclovirobuxine D in various physiological samples can be realized. The electrochemical sensor, device and method provided by the invention have a wide application prospect in quality standard research of the cyclovirobuxine D and physiological and pathological events related to the cyclovirobuxine D.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to an electrochemical sensor, a device and a method for detecting cyclovirin buxicine D. Background technique [0002] Huanwei Buxus D is a refined alkaloid extracted from Euonymus microphylla and its congeners. It has the effects of promoting qi and blood circulation, dredging collaterals and relieving pain. It is mainly used clinically for chest obstruction, heartache, and pulse knots caused by qi stagnation and blood fatigue. generation, coronary heart disease, etc. Huowei Buxusing D and its preparation Huangyangning Tablets, as a single natural product drug independently developed by China, were included in the 2000 edition of the "Chinese Pharmacopoeia". Clinical studies have shown that Cycloviral Buxicine D has strong tissue affinity, rapid uptake, long retention time in the tissue, and slow excretion. Regardless of oral or intravenous injection of Cycloviral Buxicine D, l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/48G01N27/308
Inventor 张自品郗伟艳翟佳莉张云静彭灿
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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