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Electrochemical sensor for simultaneously detecting guanine and adenine and preparation method thereof

A technology of adenine and guanine, which is applied in the field of electrochemical electrode material preparation, can solve the problems of weak electrochemical response, difficult simultaneous sensitive analysis and stable detection, etc., and achieves good stability and reproducibility, and improves the effect of selectivity.

Active Publication Date: 2019-04-09
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high oxidation potential and weak electrochemical response of G and A, it is difficult for existing electrochemical sensors to achieve simultaneous sensitive analysis and stable detection of them.

Method used

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  • Electrochemical sensor for simultaneously detecting guanine and adenine and preparation method thereof
  • Electrochemical sensor for simultaneously detecting guanine and adenine and preparation method thereof
  • Electrochemical sensor for simultaneously detecting guanine and adenine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: An electrochemical sensor for simultaneous detection of guanine and adenine, based on the composite material of copper@nickel / nitrogen, boron-doped reduced graphene oxide, the preparation method of the electrochemical sensor is:

[0018] (1) Preparation of nitrogen and boron doped reduced graphene oxide N,B-rGO by hydrothermal method: 10 mg of graphene GO was mixed with 20 ml of water, 40KHz ultrasonic treatment was performed for 10 min to 30 min, and GO was dispersed in water to form a concentration To a uniform GO suspension of 0.5 mg / mL, add dilute ammonia water with a mole fraction of 5%, adjust the pH of the suspension to 8.0, add 1.0 g of urea and 50.0 mg of boric acid to the suspension under vigorous stirring; put the mixture at room temperature Stirred at 120°C for 30 minutes, transferred to a Teflon-lined reactor at 120°C for 12 hours, then cooled in air, centrifuged at 12,000 rpm to collect N,B-rGO complexes and washed repeatedly with water and et...

Embodiment 2

[0023] Example 2: Characterization of the prepared electrochemical sensor modification process

[0024] Take the modified electrode prepared in Example 1, add 5.0 mM [Fe(CN) 6 ] 3- / 4- As a probe, the modification process of the electrode was characterized by cyclic voltammetry. Such as Figure 4 Shown: The peak current generated by the electrode after modification of nitrogen and boron doped reduced graphene oxide (N,B-rGO) increases slightly, which is due to its good conductivity. After modifying nickel / nitrogen and boron-doped reduced graphene oxide (Ni / N,B-rGO), the peak current is significantly improved due to the accelerated electron transfer efficiency on the electrode surface. After modifying copper@nickel / nitrogen and boron-doped reduced graphene oxide (Cu@Ni / N, B-rGO), the peak current is further significantly improved. Figure 4 It shows that the electrode modification process prepared in Example 1 is all successful.

Embodiment 3

[0025] Example 3: The electrochemical sensor prepared in Example 1 simultaneously detects guanine and adenine

[0026] Take the different modified electrodes prepared in Example 1 (bare electrode, nitrogen, boron doped reduced graphene oxide, nickel / nitrogen, boron doped reduced graphene oxide and copper@nickel / nitrogen, boron doped reduced graphene oxide), Electrochemical detection is carried out on the two base mixtures to be determined, and the cyclic voltammograms of guanine and adenine are obtained. After copper@nickel / nitrogen and boron doped reduced graphene oxide modified electrodes, the current signal was significantly enhanced, and the sensitivity of the sensor was greatly improved. Figure 5 It shows that the electrode modification process prepared in Example 1 is all successful, and guanine and adenine can be detected simultaneously.

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Abstract

The invention belongs to a technical field of preparation of electrochemical electrode materials, and provides an electrochemical sensor for simultaneously detecting guanine and adenine. The electrochemical sensor based on copper@nickel / nitrogen, boron doped reduction graphene oxide simultaneously detects guanine and adenine. The electrochemical sensor is based on copper@nickel / nitrogen, boron doped reduction graphene oxide composites, and prepared by modifying a surface of glassy carbon electrode. The electrochemical sensor has high sensitivity and selectivity, good stability and reproducibility, and the electrode modification process is simpler. The preparation method comprises: based on the copper@nickel / nitrogen, boron doped reduction graphene oxide composites, preparing the nitrogen,boron doped reduction graphene oxide N, B-rGO by hydrothermal method, and preparing the copper@nickel / nitrogen, boron doped reduced graphene oxide Cu@Ni / N, B-rGO; and preparing an electrode. The invention improves the sensitivity of the electrode and makes the electrode modification process simpler. The electrochemical sensor is used to construct a sensing system for simultaneous detection of guanine and adenine in adenosine, which significantly increases electrode selectivity.

Description

technical field [0001] The invention belongs to the technical field of preparation of electrochemical electrode materials, and specifically relates to an electrochemical sensor for simultaneously detecting guanine and adenine and a preparation method thereof. The electrochemical sensor is based on copper@nickel / nitrogen, boron-doped reduced graphene oxide An electrochemical sensor for the simultaneous detection of purine and adenine in human serum. Background technique [0002] Guanine (G) and adenine (A) are important purine bases in deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). They play an important role in inhibiting neurotransmitter release, coronary and cerebral circulation, maintaining heart rhythm and controlling blood flow. The presence of G and A in physiological fluids may result from enzyme dysfunction in nucleic acid metabolism or other metabolic mechanisms due to tissue degradation or dietary intake. Abnormal changes in purine bases in organisms sug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48C01B32/184B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00C01B32/184G01N27/308G01N27/48
Inventor 雷鹏周影朱瑞琦双少敏
Owner SHANXI UNIV
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