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Electrochemical immunosensor as well as preparation method and application thereof

An immunosensor, electrochemical technology, applied in scientific instruments, instruments, material analysis through electromagnetic means, etc., can solve the problems of low sensitivity, cumbersome process, modification method to be developed, etc., to achieve good stability and reproducibility, The effect of increasing selectivity

Inactive Publication Date: 2017-08-11
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional electrode modification method needs to modify the electrode surface layer by layer, the process is cumbersome, and the sensitivity is low. New modification methods need to be developed

Method used

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  • Electrochemical immunosensor as well as preparation method and application thereof
  • Electrochemical immunosensor as well as preparation method and application thereof
  • Electrochemical immunosensor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of electrochemical immunosensor with Janus particle modified glassy carbon electrode:

[0040] (1) Add 150mL of 1mM chloroauric acid solution into a flask with a reflux device, stir, heat to boiling, then quickly add 15mL of 38.8mM sodium citrate solution, and reflux for 15 minutes until the solution turns wine red . Remove the flask from the heat source, continue to stir, cool to room temperature, and store at 4°C for later use;

[0041] (2) Take hydrogen peroxide with a concentration of 30%, configure piranha solution with 70% concentrated sulfuric acid and 30% hydrogen peroxide, place the silicon chip in the solution, heat and boil at 85°C for 15 minutes, and remove impurities on the surface of the silicon chip. Take it out, rinse it with ultrapure water, and set aside;

[0042] (3) Disperse 200 μL of 2wt% aminopolystyrene microspheres in 200 μL of ethanol solution. The diluted PS microspheres were sonicated for 30s. Add ultrapure water into a glass p...

Embodiment 2

[0050] A 25nm gold layer was deposited on one side of polystyrene microspheres by vacuum thermal evaporation technology to prepare Janus particles. The prepared Janus particles were observed with a scanning electron microscope. Such as figure 2 As shown in the scanning electron microscope picture, it can be clearly seen that half of the gold layer is evaporated on the surface of the Janus particles, and the metal-PS boundary of the Janus particles forms a half-moon shape.

Embodiment 3

[0052] The surface of the glassy carbon electrode modified in Example 1 was characterized by a scanning electron microscope. Such as image 3 As shown, it can be seen from the figure that Janus particles are attached to the surface of the glassy carbon electrode and adsorb a large number of gold nanoparticles, and the surface of gold nanoparticles can immobilize a large number of ractopamine aptamers, which can greatly improve the sensitivity of the immune electrode. improvement. It shows that the aptamer / octadecanethiol Janus particle immune electrode prepared by the present invention is successfully modified.

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Abstract

The invention provides an electrochemical immunosensor as well as a preparation method and an application thereof. Janus particles with hydrophilic / hydrophobic properties are prepared with aptamer / octadecanethiol as functional groups and are used for modifying a glassy carbon electrode, and the electrochemical immunosensor is prepared. The sensor prepared with the method can be used for detecting beta-receptor agonist ractopamine. By means of the method, the sensitivity and the selectivity of the electrode are improved, so that the electrode modification process is simpler and more convenient, and the electrochemical immunosensor has good stability and reproducibility and can be applied to detection of ractopamine in human urine.

Description

technical field [0001] The invention relates to the technical field of preparation of electrochemical electrode materials, in particular to a preparation method of an electrochemical immunosensor and the application of the prepared sensor in detecting ractopamine in human urine. Background technique [0002] Electrochemical immunosensor combines the high specificity of immune reaction with the high sensitivity of electrochemical analysis. Because of its advantages of small sample consumption, high sensitivity and low cost of instruments, it has become a new method in clinical, biomedicine, environmental chemistry and food safety analysis. a powerful analytical tool. Among them, electrode modification is the key to the preparation of immunoelectrochemical sensors. The traditional electrode modification method requires layer-by-layer modification of the electrode surface, which is cumbersome and has low sensitivity. New modification methods need to be developed. [0003] Jan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/94G01N33/543G01N27/327
CPCG01N27/3277G01N27/3278G01N33/54346G01N33/9413
Inventor 周影双少敏
Owner SHANXI UNIV
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