A kind of electrochemical immunosensor based on janus particle and its preparation method and application
An immunosensor, electrochemical technology, used in scientific instruments, instruments, material analysis by electromagnetic means, etc., to achieve good stability and reproducibility, and improve the effect of selectivity
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[0033] (1) Use 70% concentrated sulfuric acid and 30% hydrogen peroxide to configure piranha solution at a volume ratio of 3:1, put the silicon chip into the solution, heat and boil at 85°C for 15 minutes, remove impurities on the surface of the silicon chip, and take it out. Rinse with ultrapure water and set aside;
[0034] (2) Disperse the aqueous solution of aminopolystyrene microspheres with a mass fraction of 2% in absolute ethanol at a volume ratio of 1:1; ultrasonically 30s the dispersed PS microspheres; add ultrapure water to a glass petri dish, and The treated PS microsphere solution is slowly added dropwise on the surface of ultrapure water, and 10 microliters of sodium dodecylsulfonate solution with a mass concentration of 2wt% is added to arrange the PS microspheres into a compact monolayer; use the first step to prepare The silicon wafers were fished out and arranged as a single layer of PS microspheres, and dried for later use;
[0035] (3) vapor-deposit a gold...
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