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A kind of electrochemical immunosensor based on janus particle and its preparation method and application

An immunosensor, electrochemical technology, used in scientific instruments, instruments, material analysis by electromagnetic means, etc., to achieve good stability and reproducibility, and improve the effect of selectivity

Active Publication Date: 2021-09-28
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there are few reports on the application of Janus particles in electrochemical detection

Method used

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  • A kind of electrochemical immunosensor based on janus particle and its preparation method and application
  • A kind of electrochemical immunosensor based on janus particle and its preparation method and application
  • A kind of electrochemical immunosensor based on janus particle and its preparation method and application

Examples

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Embodiment 1

[0033] (1) Use 70% concentrated sulfuric acid and 30% hydrogen peroxide to configure piranha solution at a volume ratio of 3:1, put the silicon chip into the solution, heat and boil at 85°C for 15 minutes, remove impurities on the surface of the silicon chip, and take it out. Rinse with ultrapure water and set aside;

[0034] (2) Disperse the aqueous solution of aminopolystyrene microspheres with a mass fraction of 2% in absolute ethanol at a volume ratio of 1:1; ultrasonically 30s the dispersed PS microspheres; add ultrapure water to a glass petri dish, and The treated PS microsphere solution is slowly added dropwise on the surface of ultrapure water, and 10 microliters of sodium dodecylsulfonate solution with a mass concentration of 2wt% is added to arrange the PS microspheres into a compact monolayer; use the first step to prepare The silicon wafers were fished out and arranged as a single layer of PS microspheres, and dried for later use;

[0035] (3) vapor-deposit a gold...

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Abstract

An electrochemical immunosensor based on Janus particles and its preparation method and application, belonging to the field of electrochemical electrode material preparation technology and food toxin detection, the purpose is to provide an electrochemical immunosensor, which is functionalized with aptamers / amino groups The Janus particles were prepared by the group and used to modify the surface of glassy carbon electrodes for the detection of ochratoxin A in red wine. The prepared electrochemical immunosensor has good stability and reproducibility, and can detect ochratoxin A in actual red wine.

Description

technical field [0001] The invention belongs to the fields of electrochemical electrode material preparation technology and food toxin detection, and in particular relates to an electrochemical immunosensor based on Janus particles and its preparation method and application. Background technique [0002] Ochratoxin A (ochratoxin, OTA) is a toxic metabolite produced by toxin-producing strains such as Aspergillus and Penicillium. It is widely found in various foods, feeds and other agricultural by-products, and mainly endangers human and animal kidneys. It was first discovered in corn, and then successively detected from grains. Not only pollute grains, wine, beer, coffee, cocoa and chocolate, Chinese herbal medicine, seasoning and other plant products and food, but also pollute animal feed very seriously. Contaminated feed is accumulated in the body after being eaten by animals, and it is not easy to be metabolized and degraded. Therefore, ochratoxin is often detected in ani...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N27/327
CPCG01N27/3275G01N33/54313
Inventor 周影杨亚娟双少敏邢洋
Owner SHANXI UNIV
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