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Indole electrochemical sensor constructed based on nitrogen-doped ordered mesoporous carbon modified electrode and application thereof

A technology for indole electrochemistry and electrode modification, which is applied in the field of electrochemical detection to achieve the effects of accelerating electron transfer, avoiding grinding and polishing, and simple fabrication and modification processes.

Active Publication Date: 2021-01-08
重庆医科大学国际体外诊断研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been reports on the detection of plasma indole by electrochemical methods, there is no report on the quantitative detection of plasma indole by using nitrogen-doped ordered mesoporous carbon modified screen-printed carbon electrodes.

Method used

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  • Indole electrochemical sensor constructed based on nitrogen-doped ordered mesoporous carbon modified electrode and application thereof
  • Indole electrochemical sensor constructed based on nitrogen-doped ordered mesoporous carbon modified electrode and application thereof
  • Indole electrochemical sensor constructed based on nitrogen-doped ordered mesoporous carbon modified electrode and application thereof

Examples

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Embodiment 1

[0038] This example proposes a plasma indole electrochemical sensor based on nitrogen-doped ordered mesoporous carbon modified electrodes, and its preparation specifically includes the following steps:

[0039] (1) Prepare the screen printing carbon electrode of the present invention:

[0040] The screen printing carbon electrode of the present invention is to sequentially print carbon paste, silver / silver chloride paste and insulating paste on a PET substrate. Specifically include the following steps:

[0041] ①Clean the PET substrate, print carbon paste on the PET substrate after drying, make working electrodes and auxiliary electrodes, and dry at room temperature;

[0042] ②Print silver paste containing silver chloride on the above-mentioned PET substrate to make a reference electrode and dry at room temperature;

[0043] ③Avoid the circular working area, print insulating paste on the above PET substrate to cover the wires;

[0044] ④The above-mentioned working electrode...

Embodiment 2

[0051] In this example, scanning electron microscopy and transmission electron microscopy were used to characterize the surface morphology and microstructure of the nitrogen-doped ordered mesoporous carbon. The results are shown in figure 2 . Figure A is a scanning electron microscope image. It can be seen from the figure that the nitrogen-doped ordered mesoporous carbon has a rod-like morphology, and is uniformly dispersed in the chitosan solution without obvious agglomeration. Figure B is a transmission electron microscope image. Nitrogen-doped ordered mesoporous carbon has a high specific surface area and abundant mesoporous channels.

Embodiment 3

[0053] This embodiment is a method for using an electrochemical sensor for detecting plasma indole, which specifically includes the following steps:

[0054] (1) Purchasing analytically pure ether for pretreatment of plasma samples;

[0055] (2) Prepare phosphate buffer solution: prepare 0.1mol L respectively -1 Sodium dihydrogen phosphate solution and disodium hydrogen phosphate solution are mixed according to a certain volume ratio to prepare a phosphate buffer solution with a pH value of 6.0;

[0056] (3) Take 300 μL of plasma sample and 600 μL of ether solution in a test tube, shake it on a shaker at 250 rpm for 18 min, then centrifuge at 13300 g and 4 °C for 5 min, and finally take the supernatant in a centrifuge tube and put Vacuum centrifugal concentrator spin dry;

[0057] (4) Reconstitute the treated extract with 300 μL of the phosphate buffer solution, vortex and mix to obtain a mixed solution;

[0058] (5) Connect the modified electrode to CHI852C electrochemical...

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Abstract

The invention relates to an indole electrochemical sensor constructed based on a nitrogen-doped ordered mesoporous carbon modified electrode and application of the indole electrochemical sensor, and belongs to the field of electrochemical detection. A compound formed by dispersing nitrogen-doped ordered mesoporous carbon in chitosan is modified on the surface of a screen-printed electrode, and because of high specific surface area and good conductivity of a nitrogen-doped ordered mesoporous carbon material as well as the characteristics of quick response, simplicity and convenience in operation and the like of the electrochemical detection method, an electrochemical sensor for detecting the plasma indole is constructed. Electrochemical detection is carried out by adopting differential pulse voltammetry, and a standard curve is drawn according to a linear relationship between an electrochemical response signal and the concentration of a to-be-detected substance, so that quantitative analysis is carried out on plasma indole. The indole electrochemical sensor for detecting the plasma indole has the advantages of simplicity and convenience in preparation, low cost, high sensitivity, strong anti-interference capability and the like.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, and in particular relates to an indole electrochemical sensor constructed on the basis of a nitrogen-doped ordered mesoporous carbon modified electrode and an application thereof. Background technique [0002] Indole is widely found in nature and has a strong smell, and its highly diluted solution can be used as a fragrance. In addition, indole is not only an important organic synthesis intermediate and an important precursor for the design and development of new drugs, but also participates in the regulation of human physiological functions as a signaling molecule. Indole is a product of tryptophan metabolized by intestinal flora in the host body, which plays an important role in maintaining intestinal health and regulating hormone secretion. On the one hand, indole can promote the barrier of intestinal epithelial cells by strengthening the tight junction between cells On the other hand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 丁敏杨可张晓清周莹蒋梦丹罗灿
Owner 重庆医科大学国际体外诊断研究院
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