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A standardized method for measuring blood physiological indexes of shellfish

A determination method and a technology for physiological indicators, which are applied in the field of marine shellfish aquaculture

Active Publication Date: 2022-05-10
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, this technology has not been involved in the field of shellfish cultivation, so the present invention applies blood gas analysis technology to the field of shellfish cultivation for the first time, and provides standard reference values ​​of blood physiological indicators of scallops, providing a method for accurate evaluation of the physiological state of shellfish. Fast and effective evaluation method

Method used

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  • A standardized method for measuring blood physiological indexes of shellfish
  • A standardized method for measuring blood physiological indexes of shellfish

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Experimental program
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Effect test

Embodiment 1

[0027] In July 2019, a total of 180 healthy scallops of different varieties, different ages, and different batches were collected from Dalian and Qingdao for analysis of blood physiological indicators. Through a large number of measurements, the blood gas standard reference values ​​of healthy scallops were established, including the following step:

[0028] 1. Temporary raising of shellfish: 180 healthy scallops (as shown in Table 1) of different varieties, different shellfish ages, and different batches were temporarily raised in the laboratory for 7 days to adapt to the environment in the laboratory;

[0029] Table 1: Shellfish Sources

[0030]

[0031]

[0032] ②Equipment debugging: Debug PL2000 blood gas electrolyte analyzer (Nanjing Pulang Medical) to make it in normal working condition;

[0033] ③Blood sampling: Take the scallops to be tested and dry them for 60 seconds to stimulate their shell opening. After the seawater in the shells has drained out, make a 5mm...

Embodiment 2

[0038] A standardized determination method for blood physiological indicators of shellfish, taking Penglaihong No. 2 Chlamys farreri from the sea area of ​​Shazikou, Qingdao, Shandong as an example, to perform standardized determination of shellfish blood physiological indicators:

[0039] (1) 40 healthy first-year-old scallops to be tested and 20 healthy second-year-old scallops to be tested were temporarily raised in the laboratory for 7 days to adapt to the environment in the laboratory;

[0040] (2) Debug PL2000 blood gas electrolyte analyzer (Nanjing Pulang Medical), make it in normal working condition, all indicators of the detection instrument are normal, and perform two-point calibration;

[0041] (3) Take 1 scallop to be tested and let it dry for 1 minute to stimulate its shell opening. After the seawater drains away, make an incision of about 5 mm in length in the center of the adductor muscle, and use a disposable syringe to absorb the shellfish blood from the incisi...

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Abstract

The invention discloses a standardized measurement method for blood physiological indexes of shellfish. By collecting blood of shellfish with a syringe and using a blood gas analyzer, the blood physiological indexes of shellfish are analyzed efficiently, quickly, with high throughput and with wide indexes. , It only takes about 3 to 5 minutes to measure each sample. And according to this method, by measuring a large number of scallops from different sources, different strains, and different shellfish ages, a standard reference value of blood physiological indicators of scallops can be provided, which can provide a basis for evaluating the physiological health status of shellfish. The invention quickly detects the blood physiological indexes of the shellfish blood, and judges the growth and life state and physiological activity of the shellfish through various indexes, and provides a feasible solution for fast, rapid and high-throughput sample measurement.

Description

technical field [0001] The invention relates to a standardized measurement method for blood physiological indexes of shellfish, which is mainly used in the measurement technology of blood physiological indexes and belongs to the field of marine shellfish cultivation. Background technique [0002] Shellfish is an important aquaculture species in my country, with a large scale of aquaculture and high economic value, and occupies an important position in the entire aquaculture industry. The distribution environment of shellfish is extensive and diverse, and its life history is complex, so the growth and development of shellfish are easily affected by the external environment. In recent years, global environmental problems such as climate warming and ocean acidification have had a serious impact on the living conditions of shellfish. At the same time, the high stocking density and small breeding population during shellfish culture have caused the degradation of germplasm. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/416G01N1/14
CPCG01N27/4166G01N1/14
Inventor 黄晓婷崔畅窦峥杨祖晶孙繁华彭程王申海陆维邢强包振民
Owner OCEAN UNIV OF CHINA
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