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Method for detecting scalp malassezia

A Malassezia and scalp technology, applied in the field of microbial detection, can solve the problems of not being able to intuitively reflect the natural state of skin lesions, changing the relative position of bacteria and skin cells, not being suitable for fast and simple dyeing, etc., so as to facilitate mobile observation and Search for relevant fields of view, facilitate staining and microscopic examination, and facilitate procurement

Inactive Publication Date: 2020-02-18
ZHUHAI EASYCARE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the collection of Malassezia samples, the methods currently used include blunt scalpel scraping method, scrubbing method, toothbrush method and extrusion method; the relatively simple method is the toothbrush method, but this method will change the specimen. The relative position of bacteria and skin cells cannot intuitively reflect the natural state of skin lesions, and it is impossible to judge the relative amount of bacteria per unit area
For sample staining, currently used methods include KOH method, DMSO-KOH method, lactate phenol cotton blue staining, Gram staining and Parker ink staining, but the above methods are suitable for clinical medicine or basic scientific research, not for Quick and easy dyeing in everyday life
In the microscopic examination of samples, at present, the samples are usually placed on ordinary glass slides for staining and microscopic examination, but the glass slides are fragile and the samples cannot be fixed, which is not convenient for staining and microscopic examination

Method used

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  • Method for detecting scalp malassezia
  • Method for detecting scalp malassezia
  • Method for detecting scalp malassezia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 standard bacterial strain Malassezia furfur staining comparison

[0051] 1. Equipment

[0052] Instrument: Leica microscope;

[0053] Consumables: hollow glass slides, transparent polyester film tape Corneofix, straws;

[0054] Reagents: 10% KOH gentian violet stain, 10% KOH Parker ink stain, cedar oil, ultrapure water.

[0055] 2. Sample

[0056] Standard strain Malassezia furfur (this strain was purchased from ATCC, Malassezia furfur is a type species of Malassezia genus; it can be used as a characteristic count strain of Malassezia spp.

[0057] 3. Steps

[0058] 1) When the standard bacterial strain Malassezia furfur was stained and counted, a ring of small colonies was picked with a sterile inoculation loop and spread on scotch tape;

[0059] 2) Staining: Add 3 drops of 10% KOH gentian violet stain or 3 drops of 10% KOH Parker ink stain to cover the sample surface with a disposable dropper on the hollow slide of the sample; stain for 30 seconds. ...

Embodiment 2

[0064] Embodiment 2 experimenter's sample dyeing comparison

[0065] In the present invention, the scotch tape sticking method is selected to collect the scalp dandruff sample, and the international commonly used Parker ink staining method is selected for Malassezia staining, and compared with the gentian violet staining method of the present invention. The specific method is as follows:

[0066] 1. Equipment

[0067] Instrument: Leica Microscope

[0068] Consumables: Cutout glass slides, clear mylar tape Corneofix, pipettes

[0069] Reagents: 10% KOH Gentian Violet Stain, 10% KOH Parker Ink Stain, Cedar Oil, Ultrapure Water

[0070] 2. Dandruff sampling

[0071] Method: scotch tape sticking method;

[0072] Sampling site: The fixed-point positioning of the head is the hairline of the forehead, the top of the head, the hairline of the back of the head and the neck, and 2cm above the left and right ears, and the sampling area is 2.25cm 2 ; Sampling was carried out at 5 sa...

Embodiment 3

[0080] Example 3 Different scalp types subject tracking detection

[0081] Three subjects with different scalp types were selected for tracking, and the sampling points were set after shampooing. The time points for dry scalp were 3h, 24h, 48h, 72h, 96h, and 130h; the time points for oily scalp were 3h, 24h, and 48h. The healthy scalp time points are 24h, 48h, and 72h; after the sampling is completed, carry out staining and microscopic examination according to the above staining steps, count the spores of Malassezia, and make the growth curve of Malassezia colonization in the scalp; The type of scalp, the type of care given and the use of products.

[0082] The specific implementation method is as follows:

[0083] 1. Equipment

[0084] Instrument: Leica microscope;

[0085] Consumables: hollow glass slides, transparent polyester film tape Corneofix, straws;

[0086] Reagents: 10% KOH gentian violet stain, cedar oil, ultrapure water.

[0087] 2. Dandruff sampling

[0088...

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Abstract

The invention provides a method for detecting scalp malassezia, and belongs to the technical field of microbiological detection. The method comprises the following steps: 1) adhering a dandruff samplefrom a fixed point on the scalp by using a transparent adhesive tape, and adhering the transparent adhesive tape with the adhered dandruff sample on a hollow glass slide; and 2) dyeing the dandruff sample with a KOH gentian violet solution, cleaning the residual dye liquor, and performing microscopic examination. By adopting the method provided by the invention, the natural state of skin lesionscan be intuitively reflected, and the relative bacterial amount per unit area is judged. In the method provided by the invention, the transparent adhesive tape with the adhered dandruff sample is adhered on the hollow glass slide to facilitate the dyeing and the microscopic examination, furthermore, the adhesive tape has high transparency, flat surface and good refractive index of microscopic examination, which is convenient for moving observation and searching for related fields during the microscopic examination. By adopting the method provided by the invention, the scalp malassezia can be detected effectively, conveniently and quickly.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a method for detecting Malassezia scalp. Background technique [0002] Malassezia is a lipophilic basidiomycete yeast that is resident on the skin of humans and warm-blooded animals. Malassezia include M.furfur, M.pachydermatis, M.sympodialis, M.globosa , M.obtuse, M.restricta and M.slooffiae etc. At present, the research on the biological characteristics, pathogenic factors, related diseases and treatment of Malassezia is more and more extensive and in-depth. [0003] In daily life, the dandruff that plagues us is closely related to Malassezia. Among the Malassezia species, Malassezia furfur became the dominant bacterial group in Malassezia scalp. Studies have shown that when dandruff is treated with anti-dandruff preparations, the number of dandruff is reduced while the number of Malassezia furfur is also reduced. Such a phenomenon speculates that Malassezi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/30G01N1/34
CPCG01N1/30G01N1/34G01N21/84
Inventor 粟雨桑何海鸥明新林周立武王德友
Owner ZHUHAI EASYCARE TECH CO LTD
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