Individualized-medication gene detection system kit for cardiovascular diseases and application of individualized-medication gene detection system kit for cardiovascular diseases

A gene detection and kit technology, which is applied in the field of genetic detection system kits for individualized drug use in cardiovascular diseases, to achieve accurate results, reduce pollution, and avoid wrong drug use

Active Publication Date: 2020-02-28
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a cardiovascular disease individualized medicine gene detection system kit and its application, which overcomes the defect that the existing cardiovascular disease gene detection kit only detects a single application direction, and the detection results provide doctors and patients with More comprehensive and reliable information, so as to make a more reasonable medication plan and ensure medication safety

Method used

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  • Individualized-medication gene detection system kit for cardiovascular diseases and application of individualized-medication gene detection system kit for cardiovascular diseases
  • Individualized-medication gene detection system kit for cardiovascular diseases and application of individualized-medication gene detection system kit for cardiovascular diseases
  • Individualized-medication gene detection system kit for cardiovascular diseases and application of individualized-medication gene detection system kit for cardiovascular diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Sample collection and DNA extraction

[0050] Collect 200 μl of blood from patients with cardiovascular disease, and use the Axygen DNA extraction kit to extract genomic DNA. For specific methods, refer to the kit instructions. DNA was quality controlled using 0.8% agarose gel electrophoresis and OD260 / 280 values.

[0051] Multiplex PCR

[0052] The multiplex PCR primer sequences of 19 SNP sites related to the metabolic pathway of cardiovascular disease therapeutic drugs use the primer sequences published in the present invention. The 19 pairs of primers were divided into two groups for multiplex PCR amplification. Group 1 includes 10 SNP sites: rs4149056, rs12248560, rs4986893, rs10306114, rs4343, rs5186, rs4244285, rs1057910, rs776746, rs2108622; Group 2 includes 9 SNP sites: rs429358, rs671, rs594918, rs5, 6 rs1801253, rs1065852, rs12041331. The PCR system uses 15μl system: 1.5mM MgCl2, 0.2mMdNTP, 2μl primer mix, 2μl DNA template, 1U Takara hot start Taq enzyme (...

Embodiment 2

[0058] Clinical genetic testing application

[0059] Sample collection and DNA extraction

[0060] 237 blood samples came from patients with cardiovascular diseases in Shanghai Songjiang District Central Hospital, including hypertension, coronary heart disease, chronic heart failure, hyperlipidemia or myocardial infarction. Please see Appendix 1 for patient information. Take 200 μl of blood, and use the Axygen DNA extraction kit to extract genomic DNA. For specific methods, refer to the kit instructions. DNA was quality controlled using 0.8% agarose gel electrophoresis and OD260 / 280 values.

[0061] Table 1 shows the age and gender distribution of patients with blood samples

[0062] information men and women upper age limit lower age limit 50～59(y) 60～69(y) 70～79(y) 80～89(y) 90～100(y) number 106 / 131 98 53 10 105 71 48 3

[0063] method evaluation

[0064] In order to type 19 SNPs at the same time, the probes for LDR detection were de...

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Abstract

The invention relates to an individualized-medication gene detection system kit for cardiovascular diseases and application of the individualized-medication gene detection system kit for the cardiovascular diseases. The kit comprises a PCR reaction system and an LDR reaction system. By combination of the multiple PCR technique and the multiple LDR technique, dual guarantee can be realized on the basis of hybridization reaction and ligation reaction, DNA purification in a detection process is avoided, and probability of contamination is reduced, so that high accuracy of results is realized. Inaddition, accuracy of PCR-LDR detection is comparable with Sanger sequencing, but PCR-LDR detection is higher in diversity and lower in cost.

Description

technical field [0001] The invention belongs to the field of gene detection kits and applications thereof, in particular to a genetic detection system kit for individualized medicine for cardiovascular diseases and applications thereof. Background technique [0002] Cardiovascular disease seriously endangers the health of all human beings and ranks first among many death factors. According to the survey, in 2016, about 270 million people in China were suffering from cardiovascular diseases. The mortality rate of cardiovascular diseases among rural residents was 309.33 / 100,000, and among urban residents was 265.11 / 100,000. The average fatality rate is above 43%. Commonly used clinical drugs for cardiovascular diseases include aspirin, clopidogrel, warfarin, nitroglycerin and statins. However, the same drug has very obvious differences in the efficacy of different people, which is mainly due to differences in genetic background. Six genes, including COX-1, GPⅡb / Ⅲa, CYP2C19,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6883C12Q1/6858C12Q2600/106C12Q2600/16C12Q2600/156C12Q2531/113C12Q2537/143C12Q2561/125
Inventor 李凯王斌肖君华周宇荀侯彦强曹辉
Owner DONGHUA UNIV
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