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Application of lipopolysaccharide in establishing human gingival fibroblast stress aging model

A fibroblast, cell aging technology, applied in animal cells, vertebrate cells, cell culture active agents, etc., can solve problems such as limited application scope, and achieve the effect of scientific and accurate evaluation mode

Pending Publication Date: 2020-03-06
GUANGDONG BOXI BIO TECH CO LTD
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Problems solved by technology

However, there are many causes of non-aging oral aging, including microorganisms, genetics, and environmental factors. The oxidative oral aging model can only reflect aging caused by some environmental factors, and its scope of application is still limited.

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  • Application of lipopolysaccharide in establishing human gingival fibroblast stress aging model
  • Application of lipopolysaccharide in establishing human gingival fibroblast stress aging model
  • Application of lipopolysaccharide in establishing human gingival fibroblast stress aging model

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Embodiment Construction

[0033] The technical solutions of the present invention will be further described below through specific embodiments. It should be clear to those skilled in the art that the embodiments are only for helping to understand the present invention, and should not be regarded as specific limitations on the present invention.

[0034] 1. Experimental method

[0035] (1) The effect of lipopolysaccharide on the viability of human gingival fibroblasts: set up blank group, control group and experimental group. Add 200 μL medium to each well of the 96-well plate blank group, inoculate 1×104 cells / well of human gingival fibroblasts in the control group and the experimental group, and add lipopolysaccharide to the experimental group, the final concentrations are 0.5 μg / mL and 5 μg / mL, respectively , 10μg / mL, 20μg / mL, placed at 37±1℃, 5±1%CO 2 , 95% relative humidity incubator, discard the old culture medium after 24 hours, wash twice with PBS, add 200 μL of 0.5 mg / mL MTT to each well, inc...

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Abstract

The invention provides an application of lipopolysaccharide in establishment of a human gingival fibroblast stress aging model, a method and the human gingival fibroblast stress aging model. The humangingival fibroblast stress aging model provided by the invention can reflect non-aging stress oral aging more comprehensively, and provides a more objective, scientific and accurate evaluation mode for efficacy evaluation of oral anti-aging products.

Description

technical field [0001] The invention belongs to the technical field of safety detection of skin care products or chemical substances, and in particular relates to the application and method of lipopolysaccharide in establishing a stress-aging model of human gingival fibroblasts and the stress-aging model of human gingival fibroblasts. Background technique [0002] Aging is a biological process of all life in nature under the interaction of genetic factors and internal and external environments. This process, from birth, development, growth to death, is a comprehensive change of the degenerative decline and disorder of body functions. When people pay attention to the aging of body functions such as skin, the aging of oral tissues has also received more attention. The structural and functional aging of oral tissues mainly manifests as exposed gums, tooth sensitivity and looseness caused by caries, dental plaque and periodontal tissue lesions. At the same time, oral care produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12Q1/02
CPCC12N5/0656G01N33/5038G01N33/5044C12N2501/052G01N2800/7009
Inventor 李乐张艳云卢永波
Owner GUANGDONG BOXI BIO TECH CO LTD
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