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Automatic focusing method for automatic microscope

An automatic focusing and microscope technology, applied in the field of microscope focusing, can solve the problems of insufficient speed and accuracy, low accuracy, etc., and achieve the effect of fast focusing speed, good adaptability and high focusing accuracy

Active Publication Date: 2020-03-06
MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST +1
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Problems solved by technology

[0009] Aiming at the deficiencies in the prior art, the present invention provides an automatic microscope auto-focus method, which solves the lack of speed and accuracy of the traditional auto-focus method. The problem of lower accuracy

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  • Automatic focusing method for automatic microscope
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Embodiment Construction

[0051] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0052] Such as Figure 1-2As shown, the present invention provides a technical solution: an automatic microscope autofocus method, the method includes the following steps:

[0053] Preprocessing step: move the focusing device on the automatic microscope to the slice, acquire the image at the current position, and calculate the sharpness of the image, set the sharpness and position of the current position as the initialization sharpness and position, and set T...

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Abstract

The invention discloses an automatic focusing method for an automatic microscope. The method comprises the following steps of: a preprocessing step of moving a focusing device on the automatic microscope onto a slide, acquiring an image of a current position, calculating definition of the image, setting the definition and a position of the current position as initial definition and position,, andsetting a preset step length and an initial direction; a searching step of searching a peak value with the preset step length and acquiring a rising edge and a falling edge; and a fitting step of fitting the peak value and the rising edge as well as the falling edge, finding out an optimal focal plane position and moving the focusing device on the automatic microscope to the position so as to complete the focusing process. By foreground region extraction, measurement of the peak value and the rising edge as well as the falling edge, parabola fitting and four-point single-peak fitting, the automatic focusing method for the automatic microscope solves the problems that a conventional automatic focusing method is insufficient in focusing speed and accuracy and particularly is lower in accuracy when focusing is carried out on a view field with an ultralow contrast or an ultra few foreground contents.

Description

technical field [0001] The present invention relates to the technical field of microscope focusing, in particular to a focusing method for a main camera, specifically a method aimed at improving the speed and accuracy of autofocus in digital slice scanning, especially when focusing on extremely low contrast or extremely low foreground objects. A revolutionary automated microscope autofocus method. Background technique [0002] Pathological diagnosis is an important basis for clinical diagnosis, and its accuracy directly affects the health and fate of patients. Traditional pathological diagnosis generally uses a microscope, and doctors observe and analyze the cells or tissues in the pathological section one by one under the microscope, and then make a diagnosis. This process is time-consuming and labor-intensive, which makes the doctor's body, especially the eyes, prone to fatigue, resulting in missed or misdiagnosed cases; for some difficult cases, it is not conducive to se...

Claims

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Application Information

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IPC IPC(8): G02B21/36G02B7/36
CPCG02B21/367G02B7/36
Inventor 陈作平
Owner MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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