Full-integrated small-scale chip type digital PCR detection system and detection method

Abstract

The invention provides a full-integrated small-scale chip type digital PCR detection system and detection method. The full-integrated small-scale chip type digital PCR detection system comprises a pump valve machine module, a temperature control module, an optical detection module, a circuit control module and a PCR detection chip, wherein the pump valve machine module comprises a chip feeding component and an air pressure driving component; the temperature control module is arranged on the chip feeding component, moves along with the PCR detection chip in parallel, and is used for controllingthe reaction temperature of the PCR detection chip; the optical detection module comprises one or more optical detection devices; a reaction reagent is injected in the PCR detection chip in advance,and blood samples and the reaction reagent are subjected to a PCR reaction in the PCR detection chip; and the circuit control module is used for transmitting an electric signal and completing the execution of a control command. The full-integrated small-scale chip type digital PCR detection system disclosed by the invention is compact in structure and easy to operate, and can effectively avoid pollution, and digital PCR amplification and detection can be completed in a short time.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, relates to a PCR detection system and a detection method, in particular to a fully integrated miniaturized chip-type digital PCR detection system and a detection method. Background technique [0002] The invention of in vitro polymerase chain reaction (Polymerase Chain Reaction, PCR) makes in vitro DNA amplification possible. Qualitative analysis of DNA amplification products can be achieved by agarose gel electrophoresis after PCR amplification. With the increasing demand for quantitative DNA detection, traditional PCR based on electrophoretic analysis is gradually replaced by quantitative PCR (quantitive PCR, qPCR) analysis, that is, real-time fluorescent quantitative PCR (Real-time quantitative PCR, RT-qPCR). However, fluorescent quantitative PCR generally uses PCR tubes to react and collect fluorescence. All nucleic acid templates exist in the same reaction system, and non-spe...

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Problems solved by technology

[0007] However, these digital PCR instruments currently have some disadvantages, such as complex operating procedures, generally requiring three separate devices to complete droplet generation, nucleic acid amplification, and result detection; in addition, the split design needs to be operated by professionals, increasing the Reduced labor costs; the multi-step sample transfer operation caused by the split design is prone to contamination, which makes the test results lose credibility; fully integrated, user-friendly integrated design can meet the detection needs of various scenarios to the greatest extent

The above reasons limit the development of digital PCR instruments and the further extensive application of digital PCR technology to a certain extent.

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