Bacterial inhibitor and preparation method and application thereof
A technology of bacterial inhibitor and staphylococcus, applied in the field of microorganisms, can solve the problems of limited bacterial inhibitory effect, complicated preparation process, etc., and achieve the effects of easy culture and material acquisition, simple production steps and wide distribution.
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Embodiment 1
[0033] In this example, three bacterial suspensions were prepared.
[0034] Preparation of liquid medium: Take 5 g of yeast extract, 10 g of tryptone, and 10 g of sodium chloride, add distilled water, stir on a magnetic stirrer until completely dissolved, and set the volume to 1000 mL. Sterilize at 121°C for 20 minutes. After sterilization, store in a 4°C refrigerator for later use.
[0035] Take 50 μL of methicillin-sensitive Staphylococcus aureus ATCC25933, Klebsiella pneumoniae ATCC13883, and Pseudomonas aeruginosa ATCC27853 stored in glycerol, add them to the sterilized liquid medium above, and place in a 50 mL conical In the flask, shake culture at 37°C for 12 h in a constant temperature shaker. Store in a 4°C refrigerator for later use.
Embodiment 2
[0037] In this example, 11 kinds of bryophyte extracts were prepared.
[0038] Collect fresh white hair moss, creeping moss, whip moss, East Asian blond moss, twisted leaf moss, knotty stem moss, clustered true moss, spiny juniper moss, multiform small moss, straight leaf cotton moss, thin Selaginella albicans, put it in a plastic bag and bring it back indoors, and store it in a dry place after natural air drying. The bryophytes are cleaned to obtain green bryophytes, washed three times with tap water and three times with distilled water, air-dried under natural conditions, and stored in ziplock bags.
[0039]Weigh 10 g of the above-mentioned bryophytes respectively, and pulverize them with a tissue grinder under liquid nitrogen conditions. Accurately weigh 5 g of the powder, add it to 100 mL of 95% ethanol, and place it on a constant temperature shaker at 37 °C for 24 h. Centrifuge at 1500 rpm for 10 min at 4°C and collect the supernatant. The residue was extracted twice w...
Embodiment 3
[0041] In this embodiment, antibacterial test I was carried out.
[0042] Take 200 μL of the series of extracts obtained in Example 2, respectively, and add them into sterilized liquid culture medium to prepare a total volume of 5 mL per tube. Each series was respectively inoculated with methicillin-sensitive Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa, and the amount of inoculation was 20 μL of the bacterial suspension prepared in Example 1. The liquid culture medium used was the blank control group, and each series was repeated 3 times.
[0043] Place in a constant temperature shaker at 37°C for 6 h, and use Nanodrop for colorimetric determination with an absorption wavelength of 600 nm (this determination method is simpler and more accurate than the traditional plate counting method). The average value was obtained from three repetitions. The results are shown in Table 1, Table 2 and Table 3 (Table 1 is the inhibitory result to methicillin-sen...
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