Preparation method of white lotus ester extract

The technology of extract and white lotus is applied in the field of preparation of white lotus-like ester extract, which can solve the problems of affecting natural active ingredients, affecting the efficacy of skin care products, polluting the extract, etc., so as to improve skin aging skin quality and remove free radicals. , the effect of eliminating allergic factors

Inactive Publication Date: 2020-04-17
费小英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional plant extraction methods mostly use solvent extraction, which has polluted the extract in essence. Long-term use will mo...

Method used

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  • Preparation method of white lotus ester extract
  • Preparation method of white lotus ester extract
  • Preparation method of white lotus ester extract

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preparation example Construction

[0015] When the present invention is implemented, a kind of preparation method of white lotus flower ester extract, its preparation method comprises the following steps:

[0016] (1) White lotus flowers are picked at the end of June, and the flowers with stamens are refrigerated at 4°C for 24 hours;

[0017] (2) Take out the fresh flowers refrigerated for 24 hours, peel off the sepals, paste them into the base of the receptacle, cut off the pedicels, and weigh 2000g;

[0018] (3) Add 3 times the volume of distilled water at 100°C, soak for 5-6 hours, filter through gauze, reduce the dregs in the same way and extract twice, and combine the clear liquid for three times;

[0019] (4) Add the clear liquid and medicinal residues into the distillation device, directly heat and distill, collect 500ml of distilled liquid, add 500ml of distilled water, continue to collect 500ml of distilled liquid, and combine the distilled liquid;

[0020] (5) Concentrate the extracted distilled supe...

Embodiment 1

[0024] Effect of Ester Extract from White Lotus on Tyrosinase Activity

[0025] In the human body, tyrosinase is mainly found in the melanocytes of the epidermal cells of the skin. The synthesis of melanin by melanocytes is a self-stabilizing skin protection mechanism of the body. In the process of skin melanin biosynthesis, if no tyrosinase participates in the synthetic reaction, the synthesis reaction will become very slow, so the activity of tyrosinase is positively correlated with the formation of melanin. This test uses tyrosinase as a substrate, from potato The crude solution was prepared in , and arbutin was used as a positive control.

[0026] 1. Preparation of tyrosinase solution

[0027] Peel and wash the potatoes, pre-cool them at 4°C for about 4 hours, peel and cut into cubes, cover them with plastic wrap, place them in a freezer at -15°C for 24 hours, and add 4°C pre-cooled potatoes at a ratio of 1:19 (w:v). Sodium phosphate buffer solution was made into a homo...

Embodiment 2

[0053] Study on Antioxidant Activity of Ester Extract from White Lotus Flower

[0054] Determination of free radical scavenging ability

[0055] Take a 10ml colorimetric tube, add 2ml of ph7.4 phosphate buffer solution, 1.5mmol / L o-phenanthroline solution, 1.5ml7.5mmol / L ferrous ammonium sulfate solution, 1ml, shake well, and keep away from light at room temperature After reacting for 60 minutes, continuously measure the absorbance in the range of 400-600nm to determine the maximum absorption wavelength.

[0056] Determination method for scavenging free radicals

[0057] Take nine 10ml colorimetric tubes, add 2ml of pH7.4 phosphate buffer solution, 1.5ml of 1.5mmol / L o-phenanthroline solution, 1ml of 7.5mmol / L ferrous ammonium sulfate solution, and add different concentrations of white lotus Add 1ml of ester extract, then add 1ml of 0.01% hydrogen peroxide solution and dilute to the mark with distilled water. React at a constant temperature of 37°C for 60 minutes and measur...

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Abstract

The invention discloses a preparation method of a white lotus ester extract. The preparation method comprises the following steps: (1) picking and refrigerating for 24 hours; (2) peeling off the sepals, and weighing the material; (3) adding water and soaking; (4) heating and distilling; (5) performing vacuum concentration; and (6) centrifuging, precipitating and dehydrating to obtain the white lotus ester extract. Compared with the prior art, the method has the advantages that the white lotus ester extract is rich in plant polysaccharide, brass, alkaloid and other active ingredients. The invention belongs to the technical field of biology, and discloses the preparation method of a ganoderma atrum white lotus ester extract, which preliminarily tests the functions of nourishing and repairing, stimulating blood circulation, eliminating allergic factors, strengthening the natural health-care detoxification function of skin, scavenging free radicals, resisting oxidation, whitening and effectively improving the aged skin.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of white lotus flower ester extract. Background technique [0002] Since ancient times, white lotus has been known as Ganoderma lucidum in water, and white lotus is also one of the earliest plants among angiosperms. The noble character of white lotus "coming out of silt but not stained, clean and clear but not demonic" has always been sung by poets and inkmen. However, there are few researches and reports on its bioactive components at home and abroad. Most of the traditional plant extraction methods use solvent extraction, which has already polluted the extract. Long-term use will more or less cause skin allergies, and even affect the natural active ingredients and affect the effectiveness of skin care products. Contents of the invention [0003] The technical problem to be solved in the present invention is to provide a method for extracting the ester-like ...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/9789A61K2800/782A61Q19/00A61Q19/005A61Q19/02A61Q19/08
Inventor 费小英
Owner 费小英
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