A red-yellow-blue three fluorescence emission sensor and its preparation and application

A fluorescence emission and sensor technology, applied in the field of analytical chemistry and rapid detection, can solve the problems of lack of accuracy and precision of visual detection results, limited fluorescence color change range of dual fluorescence emission sensors, etc., to overcome the narrow color evolution range and price. The effect of low cost and simple preparation process
CN111077124BActive Publication Date: 2022-08-02YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
Publication Date
2022-08-02

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Abstract

The invention belongs to the fields of analytical chemistry and rapid detection, and in particular relates to a red-yellow-blue three fluorescence emission sensor and its preparation and application in accurate visual detection of alkaline phosphatase. The sensor is composed of manganese dioxide nanosheets, o-phenylenediamine, red fluorescent quantum dots and a buffer solution; or, the sensor is composed of manganese dioxide nanosheets, o-phenylenediamine, red fluorescent quantum dots and a buffer solution containing a substrate, Wherein the substrate is L-ascorbic acid-2-phosphate. The red-yellow-blue three-fluorescence emission sensor of the present invention can be excited by the same wavelength, and its three different wavelengths of fluorescence can exhibit yellow fluorescence peak reduction, red fluorescence peak enhancement and blue fluorescence for alkaline phosphatase whose activity gradually increases The peak is enhanced, and the overall fluorescence color changes to "yellow-orange-red-purple" for visual detection of the target content. The target can also be determined by measuring the fluorescence intensity at three different emission wavelengths and using the intensity change as a signal parameter. content.
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Description

technical field

[0001] The invention belongs to the field of analytical chemistry and rapid detection, and in particular relates to a red-yellow-blue three fluorescence emission sensor and its preparation and application in accurate visual detection of alkaline phosphatase. Background technique

[0002] Nowadays, rapid visual inspection plays an important role in environmental inspection, food safety, clinical diagnosis and other fields. The rapid visual detection of a large number of samples to be tested, combined with the accurate detection of suspicious samples by large-scale instruments (such as high performance liquid chromatography), often constitute a complete detection process, which not only saves the money and time spent on detection, but also ensures the reliability of the detection results. Therefore, rapid visual detection methods with high sensitivity and high selectivity are often required.

[0003] Fluorescence sensors have been widely developed and applied ...

Claims

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