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A red-yellow-blue three fluorescence emission sensor and its preparation and application

A fluorescence emission and sensor technology, applied in the field of analytical chemistry and rapid detection, can solve the problems of lack of accuracy and precision of visual detection results, limited fluorescence color change range of dual fluorescence emission sensors, etc., to overcome the narrow color evolution range and price. The effect of low cost and simple preparation process

Active Publication Date: 2022-08-02
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Then, by observing the CIE1931 chromaticity diagram, it can be seen that the range of fluorescence color changes that the constructed dual fluorescence emission sensor can provide is still limited, resulting in a lack of certain accuracy and precision in the visual detection results.

Method used

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  • A red-yellow-blue three fluorescence emission sensor and its preparation and application
  • A red-yellow-blue three fluorescence emission sensor and its preparation and application
  • A red-yellow-blue three fluorescence emission sensor and its preparation and application

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Preparation of the red-yellow-blue triple fluorescence emission sensor:

[0038] The sensor is composed of manganese dioxide nanosheets, o-phenylenediamine, red fluorescent quantum dots and buffer; the dosage of each substance is: 100 μL manganese dioxide nanosheets (0.8 mg·mL -1 ), 50μL o-phenylenediamine (0.3mol·L -1 ), 20 μL red fluorescent quantum dots; 100 μL phosphate buffer (0.1 mol·L -1 , pH 8.0). And add 750 μL water to volume; when testing samples, add 750 μL sample to volume.

[0039] Or, the sensor is composed of manganese dioxide nanosheets, o-phenylenediamine, red fluorescent quantum dots and a buffer solution containing the substrate L-ascorbic acid-2-phosphate; wherein, the amount of each substance is: 100 μL manganese dioxide nanosheets (0.8 mg·mL -1 ), 50μL o-phenylenediamine (0.3mol·L -1 ), 10 μL red fluorescent quantum dots; 100 μL phosphate buffer (0.1 mol·L -1 , pH 8.0, containing 10 mML-ascorbic acid-2-phosphate). And add 750 μL water to vo...

Embodiment 2

[0043] Accurate visual detection of ascorbic acid using the above sensors:

[0044] see figure 1 : First, 750 μL of ascorbic acid with different concentrations (0, 0.5, 1, 5, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, 90 and 100 μmol L -1 ) with 100 μL of manganese dioxide nanosheets (0.8 mg·mL -1 ) for 10 minutes, followed by adding 100 μL of phosphate buffer (0.1 mol·L -1 , pH 8.0) and 50 μL o-phenylenediamine (0.3 mol·L -1 ) in a 40°C water bath for 60 minutes. Finally, 20 μL of red fluorescent quantum dots were added, mixed well, and the fluorescence emission spectrum was recorded using a fluorometer at an excitation wavelength of 365 nm and a slit width of 10 / 10 nm, and the corresponding fluorescence images were taken under a 365 nm UV lamp. At the same time, using the same method as above, without adding red fluorescent quantum dots in the preparation process, a yellow-blue dual fluorescent emission sensor was obtained as a control.

[0045] Depend on figure 2 A show...

Embodiment 3

[0047] Accurate visual detection of alkaline phosphatase using the above sensors:

[0048] see figure 1 : First mix 750 μL of alkaline phosphatase with different activities (0, 0.1, 1, 5, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, 90 and 100 mU / mL) with 100 μL L-ascorbic acid-2-phosphate (10mM, dissolved in 0.1mol·L -1 , pH 8.0 phosphate buffer) in a 37°C water bath for 60 minutes, followed by adding 100 μL of manganese dioxide nanosheets (0.8 mg·mL -1 ) and reacted for 10 minutes, followed by adding 50 μL of o-phenylenediamine (0.3 mol·L -1 ) in a 40°C water bath for 60 minutes. Finally, 10 μL of red fluorescent quantum dots were added, mixed well, and the fluorescence emission spectrum was recorded using a fluorometer at an excitation wavelength of 365 nm and a slit width of 10 / 10 nm, and the corresponding fluorescence images were taken under a 365 nm UV lamp. At the same time, using the same method as above, without adding red fluorescent quantum dots in the preparation pr...

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Abstract

The invention belongs to the fields of analytical chemistry and rapid detection, and in particular relates to a red-yellow-blue three fluorescence emission sensor and its preparation and application in accurate visual detection of alkaline phosphatase. The sensor is composed of manganese dioxide nanosheets, o-phenylenediamine, red fluorescent quantum dots and a buffer solution; or, the sensor is composed of manganese dioxide nanosheets, o-phenylenediamine, red fluorescent quantum dots and a buffer solution containing a substrate, Wherein the substrate is L-ascorbic acid-2-phosphate. The red-yellow-blue three-fluorescence emission sensor of the present invention can be excited by the same wavelength, and its three different wavelengths of fluorescence can exhibit yellow fluorescence peak reduction, red fluorescence peak enhancement and blue fluorescence for alkaline phosphatase whose activity gradually increases The peak is enhanced, and the overall fluorescence color changes to "yellow-orange-red-purple" for visual detection of the target content. The target can also be determined by measuring the fluorescence intensity at three different emission wavelengths and using the intensity change as a signal parameter. content.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and rapid detection, and in particular relates to a red-yellow-blue three fluorescence emission sensor and its preparation and application in accurate visual detection of alkaline phosphatase. Background technique [0002] Nowadays, rapid visual inspection plays an important role in environmental inspection, food safety, clinical diagnosis and other fields. The rapid visual detection of a large number of samples to be tested, combined with the accurate detection of suspicious samples by large-scale instruments (such as high performance liquid chromatography), often constitute a complete detection process, which not only saves the money and time spent on detection, but also ensures the reliability of the detection results. Therefore, rapid visual detection methods with high sensitivity and high selectivity are often required. [0003] Fluorescence sensors have been widely developed and applied ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/643G01N21/6408G01N2021/6432G01N2021/6421
Inventor 李金花杨倩陈令新李登贞李博伟王晓艳付龙文
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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