Method for detecting concentration of metal elements in aqueous humor in combination with flow injection and inductively coupled plasma mass spectrometer
A metal element and inductive coupling technology, applied in the field of medicine, can solve the problem of low sensitivity, achieve the effect of reducing wall hanging, eliminating matrix effect and signal drift
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[0028] Example 1:
[0029] 1. Collection and storage of aqueous humor
[0030] During the operation, perform routine disinfection and draping and other steps. Before the invasive operation of the anterior chamber, puncture the anterior chamber with a disposable sterile 1mL syringe, collect 100-150μl of aqueous humor sample, and transport it to the laboratory in an ice box , Transfer to a 1.5ml sterile EP tube under aseptic conditions in a biological safety cabinet, seal with a parafilm, quickly freeze in liquid nitrogen, and store in a refrigerator at -80°C. The aqueous humor samples were sent to the testing center of Sun Yat-Sen University to be tested by FI-ICP-MS. Each sample was tested in 2 parallel samples, and the average of the two measurements was finally taken. Take single-blind operation.
[0031] 2. Pretreatment of aqueous humor detection
[0032] A. Take 50μl aqueous humor sample in a 1.5mL sterile EP tube (the blank sample of the whole procedure is replaced with 50μl Mi...
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