The invention discloses a method for detecting concentration of metal elements in aqueous humor in combination with flow injection and an inductively coupled plasma mass spectrometer. The method comprises the following steps of first, taking an aqueous humor sample; then, diluting the aqueous humor sample, and adding Triton, methanol, HNO3 and internal standard elements to obtain a diluent; and finally, injecting, by using the flow injection, the sample into the inductively coupled plasma mass spectrometer for determining the concentration of the metal elements to be detected. According to themethod, aqueous humor detection pretreatment is adopted for solving the problem that the aqueous humor sample has a wall hanging phenomenon in a sample injecting pipeline, so that the wall hanging ofthe sample in the sample injecting pipeline is greatly reduced. Moreover, by adding the internal standard elements, namely, manually selecting and adding the elements, namely, Sc, In and Ge, that arevery rare in the natural environment, the solution sample volume is standardized, and a matrix effect and signal drift are eliminated. In addition, the values to be set of the relevant parameters ofan instrument are found out, the concentration of trace metal elements in the aqueous humor is successfully detected by applying FI-ICP-MS for the first time, and taking Zn as an example, the detection limit is as low as 2.00 ug/L. The method for detecting the concentration of the metal elements in the aqueous humor in combination with the flow injection and the inductively coupled plasma mass spectrometer provides a model for the detection of the trace metal elements in the aqueous humor.